Colletotrichum gloeosporioides is a main pathogen causing rubber anthracnose, which brings huge economic loss to natural rubber industry. Oligopeptide transporter (OPT) is a kind of peptide transport protein, which usually exhibits various biological functions in fungi. In this study, an oligopeptide transporter gene CgOPT2 is identified from the rubber anthracnose fungus C. gloeosporioides as a homolog of OPT2 in Saccharomyces cerevisiae. CgOPT2 encodes a 990-amino acid protein, which has an OPT domain harboring 15 transmembrane domains. The gene knock-out mutant of CgOPT2 had reduced vegetative growth on PDA and CM media, and was more sensitive to H₂O₂, SDS and Congo Red than the wild-type strain. Moreover, the mutant had obviously lagged conidia germination and attenuated virulence on rubber leaves. Transcriptome analysis showed CgOPT2 could affect the expression of many genes involved in cellular transport, amino acid metabolism and signal transduction, etc. In conclusion, CgOPT2 is an important oligopeptide transporter of C. gloeosporioides, which participates in the process of vegetative growth, cell wall integrity, oxidative stress, conidia germination and pathogenicity.

炭疽菌是引起橡胶炭疽病的主要病原体，给天然橡胶工业带来巨大的经济损失。寡肽转运蛋白（OPT）是一种肽转运蛋白，通常在真菌中表现出多种生物学功能。在这项研究中，寡肽转运蛋白基因CgOPT2从橡胶炭疽病菌鉴定炭疽菌作为同源物OPT2在酿酒酵母。CgOPT2编码一个990个氨基酸的蛋白质，该蛋白质具有一个包含15个跨膜结构域的OPT结构域。CgOPT2的基因敲除突变体减少了PDA和CM培养基上的营养生长，并且对H ₂更加敏感O ₂，SDS和刚果红比野生型菌株强。此外，该突变体在分生孢子上明显滞后分生孢子，并且在橡胶叶上的毒力减弱。转录组分析表明CgOPT2可能影响参与细胞运输，氨基酸代谢和信号转导等。在许多结论基因的表达，CgOPT2是一个重要的寡肽转运蛋白炭疽菌，其参与植物生长的过程中，细胞壁完整性，氧化应激，分生孢子萌发和致病性。