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Physiological and morphological traits associated with germinative and reproductive stage of garden orache (A. hortensis L. var. rubra) under water stress
Chemical and Biological Technologies in Agriculture ( IF 6.6 ) Pub Date : 2021-05-14 , DOI: 10.1186/s40538-021-00218-7
S. Sai Kachout , S. BenYoussef , A. Ennajah , S. Abidi , A. Zoghlami

Drought is a major problem limiting the growth and development of plants in the world and especially in Tunisia. Halophytes constitute a renewable wealth and they offer great flexibility with regard to abiotic stresses, and they are evaluated for their ecological and potential food use. The proposed work identifies the response of Atriplex hortensis var. rubra to the germinal stage and the reproductive stage under a deficient water regime to measure the drought resistance of this plant that has very interesting forage production abilities. The morphological and water parameters are used to characterize the physiological response of this species to the effects of water deficit. For the germination test, four levels of osmotic potential caused by PEG-6000 solutions at different levels of water potential (− 0.1, − 0.5, − 1.0, − 1.5 MPa) were adopted in seed of A. hortensis germination media. The methodology adopted in the second experiment is based on the cultivation of potted plants stored in a semi-controlled greenhouse at flowering stage. The water deficit was imposed on the plants by watering stop for a week, and the control plants are subjected to a water regime maintained irrigated at 100% of the capacity in the field. Drought tolerance was scored 30 days after the drought stress commenced based on the number of branches and leaf, dry biomass, relative water content, leaf water potential, and nitrogen content. No significant difference was observed in germination rates for all PEG concentrations throughout the experiment which are still close to 60%. The results obtained for the second experiment show a high tolerance of A. hortensis under water stress. Drought induced decreases in two physiological parameters, the number of branches and leafs, and the relative water content of annual Atriplex. Heatmap and PCA data revealed that physiological parameters are more sensitive than morphological parameters in distinguishing the control and drought treatments. Indeed, the orache is distinguished by a great ability to retain water potential after a month of stress. Thus, height, number of branches, leaf and shoot dry weight, and percentage of nitrogen were significantly similar for controls and stressed for A. hortensis. On the other hand, measured root length and basic and midday water potential show significant variability between controls and stressors. In addition, these results highlight the importance of the resistance of Atriplex halophyte forage to drought.

中文翻译:

生理与花园orache的发芽和生育阶段相关的形态性状(A.菠菜L.变种杨梅水分胁迫下)

干旱是限制世界上尤其是突尼斯植物生长和发育的主要问题。盐藻构成了可再生的财富,它们在非生物胁迫方面具有极大的灵活性,并且对其生态和潜在的食品用途进行了评估。拟议的工作确定了滨藜的反应。在水分缺乏的情况下,将风疹移至生发期和生殖期,以测量具有非常有趣的草料生产能力的这种植物的抗旱性。形态学和水分参数用于表征该物种对缺水影响的生理反应。对于发芽测试,在不同水势水平下(-0.1,-0.5,-1.0,-1),由PEG-6000溶液引起的四个渗透势水平。(5 MPa)被采用在山楂种子发芽培养基的种子中。在第二个实验中采用的方法是基于在开花期将植物存放在半控制温室中的盆栽植物的栽培。通过停水一周来对植物施加缺水,并且对对照植物进行维持100%田间灌溉量的水处理。在干旱胁迫开始后30天,根据枝条和叶片的数量,干生物量,相对含水量,叶片水势和氮含量对耐旱性进行评分。在整个实验中,所有PEG浓度(仍接近60%)的发芽率均未观察到显着差异。从第二个实验获得的结果表明,在水分胁迫下,锦葵科植物具有较高的耐受性。干旱导致两个生理参数的下降,即枝叶数和一年生滨藜的相对含水量。热图和PCA数据表明,在区分控制和干旱处理方面,生理参数比形态参数更敏感。的确,在一个月的压力之后,该Orache的强大功能便是能够保留水势。因此,对照组的高度,枝条数,叶和苗的干重以及氮的百分比与拟南芥非常相似,并且受到胁迫。另一方面,测得的根长,基本和中午水势显示,对照组和胁迫源之间存在显着差异。此外,这些结果凸显了滨藜植物盐生植物饲料对干旱的抗性的重要性。以及一年生滨藜的相对含水量。热图和PCA数据表明,在区分控制和干旱处理方面,生理参数比形态参数更敏感。的确,在一个月的压力之后,该Orache的强大功能便是能够保留水势。因此,对照组的高度,枝条数,叶和苗的干重以及氮的百分比与拟南芥非常相似,并且受到胁迫。另一方面,测得的根长,碱性和中午水势显示控件和压力源之间存在显着差异。此外,这些结果凸显了滨藜植物盐生植物饲料对干旱的抗性的重要性。以及一年生滨藜的相对含水量。热图和PCA数据表明,在区分控制和干旱处理方面,生理参数比形态参数更敏感。的确,在一个月的压力之后,该Orache的强大功能便是能够保留水势。因此,对照组的高度,枝条数,叶和苗的干重以及氮的百分比与拟南芥非常相似,并且受到胁迫。另一方面,测得的根长,碱性和中午水势显示控件和压力源之间存在显着差异。此外,这些结果凸显了滨藜植物盐生植物饲料对干旱的抗性的重要性。热图和PCA数据表明,在区分控制和干旱处理方面,生理参数比形态参数更敏感。的确,在一个月的压力之后,该Orache的强大功能便是能够保留水势。因此,对照组的高度,枝条数,叶和苗的干重以及氮的百分比与拟南芥非常相似,并且受到胁迫。另一方面,测得的根长,碱性和中午水势显示控件和压力源之间存在显着差异。此外,这些结果凸显了滨藜植物盐生植物饲料对干旱的抗性的重要性。热图和PCA数据表明,在区分控制和干旱处理方面,生理参数比形态参数更敏感。的确,在一个月的压力之后,该Orache的强大功能便是能够保留水势。因此,对照组的高度,枝条数,叶和苗的干重以及氮的百分比与拟南芥非常相似,并且受到胁迫。另一方面,测得的根长,碱性和中午水势显示控件和压力源之间存在显着差异。此外,这些结果凸显了滨藜植物盐生植物饲料对干旱的抗性的重要性。因此,对照组的高度,枝条数,叶和苗的干重以及氮的百分比与拟南芥非常相似,并且受到胁迫。另一方面,测得的根长,碱性和中午水势显示控件和压力源之间存在显着差异。此外,这些结果凸显了滨藜植物盐生植物饲料对干旱的抗性的重要性。因此,对照组的高度,枝条数,叶和苗的干重以及氮的百分比与拟南芥非常相似,并且受到胁迫。另一方面,测得的根长,基本和中午水势显示,对照组和胁迫源之间存在显着差异。此外,这些结果凸显了滨藜植物盐生植物饲料对干旱的抗性的重要性。
更新日期:2021-05-14
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