The aim of this work was to find the correlation between the content of ergosterol in fungi membrane and the action of the hop essential oil, myrcene and humulene on its properties. To reach this goal, the monolayers and bilayers composed of phosphatidylcholine, phosphatidyethanol amine and ergosterol, differing in the concentration of sterol, were used as model membrane systems. The impact of the essential oil and its major terpenes on one component ergosterol film was also investigated.

It was found that pure isolated terpenes, in contrast to the hop oil being the mixture of them, do not incorporate into pure ergosterol membrane, however, they cause the loss of monolayer material from the interface. These results are in contrast to the effect of these terpenes on phospholipid films reported previously and they may suggest a strong effect of ergosterol on the behavior of terpenes in the mixed systems. Surprisingly, for model membranes, the effect of myrcene was qualitatively similar to the effect of the hop oil and ergosterol was found to regulate the incorporation of both these substances into the film. In contrast, very strong correlation between ergosterol content and the action of humulene was found. Namely, the ability of humulene to change model membrane properties was found to increase with ergosterol concentration. Additionally, the differentiating effect of ergosterol on humulene action in membranes was much more pronounced than for myrcene or the hop oil. Interestingly, at the highest ergosterol level the influence of humulene was even stronger than the effect of the hop oil. This is very important finding suggesting that ergosterol may regulate the sensitivity of particular membrane to the impact of humulene. Summarizing, ergosterol substantially differentiates the effect of the hop oil, myrcene and humulene on the lipid systems and it can be the molecule important for antifungal effect of the essential oil and terpenes.

这项工作的目的是寻找真菌膜中麦角甾醇的含量与啤酒花精油、月桂烯和葎草烯对其性质的作用之间的相关性。为了达到这个目标，由磷脂酰胆碱、磷脂酰乙醇胺和麦角甾醇组成的单层和双层，甾醇浓度不同，被用作模型膜系统。还研究了精油及其主要萜烯对单组分麦角甾醇膜的影响。

发现纯分离萜烯与作为它们混合物的啤酒花油不同，不会结合到纯麦角甾醇膜中，然而，它们会导致单层物质从界面上损失。这些结果与之前报道的这些萜烯对磷脂膜的影响形成对比，它们可能表明麦角甾醇对混合系统中萜烯的行为有很强的影响。令人惊讶的是，对于模型膜，月桂烯的作用与啤酒花油的作用在性质上相似，并且发现麦角甾醇调节这两种物质向膜中的结合。相比之下，发现麦角甾醇含量与葎草烯的作用之间存在很强的相关性。即，发现葎草烯改变模型膜特性的能力随着麦角甾醇浓度而增加。此外，麦角甾醇对膜中葎草烯作用的区分作用比月桂烯或啤酒花油明显得多。有趣的是，在最高麦角甾醇水平下，葎草烯的影响甚至强于啤酒花油的影响。这是一个非常重要的发现，表明麦角甾醇可能调节特定膜对葎草烯影响的敏感性。总之，麦角甾醇显着区分了酒花油、月桂烯和葎草烯对脂质系统的影响，它可能是对精油和萜烯的抗真菌作用很重要的分子。在最高麦角甾醇水平下，葎草烯的影响甚至强于啤酒花油的影响。这是一个非常重要的发现，表明麦角甾醇可能调节特定膜对葎草烯影响的敏感性。总之，麦角甾醇显着区分了酒花油、月桂烯和葎草烯对脂质系统的影响，它可能是对精油和萜烯的抗真菌作用很重要的分子。在最高麦角甾醇水平下，葎草烯的影响甚至强于啤酒花油的影响。这是一个非常重要的发现，表明麦角甾醇可能调节特定膜对葎草烯影响的敏感性。总之，麦角甾醇显着区分了酒花油、月桂烯和葎草烯对脂质系统的影响，它可能是对精油和萜烯的抗真菌作用很重要的分子。