Bt crops are the most important of the commercialised transgenic plants which were developed to combat destructive pests. Colorado potato beetle (CPB) is considered as a dangerous threat to potato production. In this study transgenic potato plants expressing cry3A gene were generated using Agrobacterium mediated transformation to combat the pest damage. Polymerase chain reaction (PCR) analysis confirmed the presence of transgenes in 40 out of 110 putative transgenic lines. One to three copies of cry3A gene were identified in selected transgenic lines using southern blot analysis. Moreover, immunostrip test confirmed the expression of Cry3A protein in the leaves of transgenic potato plants. Bioassay analysis with the first instar of CPB larvae indicated 100% mortality, significantly lower leaf damage and less insect biomass accumulation for transgenic lines (PC3-3, PC3-4, PC3-5, PC3-8 and PC3-13) in comparison to control plants. The results indicated that Cry3A protein expression could efficaciously increase the resistance to CPB in transgenic potato cv. Agria which is recommended for controlling CPB damage and to prevent its spread in potato cultivation regions. Moreover, these transgenic lines can be used as cultivar or germplasm to breed new cultivars.

Bt作物是为对抗破坏性害虫而开发的商业化转基因植物中最重要的。科罗拉多马铃薯甲虫（CPB）被认为是对马铃薯生产的危险威胁。在这项研究中，表达表达cry3A基因的转基因马铃薯植株是利用农杆菌介导的转化方法来对抗害虫的。聚合酶链反应（PCR）分析证实了110个推定的转基因品系中有40个存在转基因。1-3份cry3A副本使用Southern印迹分析在选定的转基因品系中鉴定了该基因。此外，免疫条测试证实了Cry3A蛋白在转基因马铃薯植株叶片中的表达。与CPB幼虫的第一龄幼虫进行的生物分析表明，与之相比，转基因品系（PC3-3，PC3-4，PC3-5，PC3-8和PC3-13）的死亡率为100％，叶片损伤显着降低，昆虫生物量积累较少。控制植物。结果表明，Cry3A蛋白的表达可以有效地提高转基因马铃薯cv对CPB的抗性。建议使用Agria来控制CPB损害并防止其在马铃薯栽培区传播。而且，这些转基因品系可以用作栽培品种或种质来育种新品种。