The osteoblast cell growth and cancer cell inhibition of titanium (Ti) metal implant coated with polyvinyl alcohol (PVA) reinforced hydroxyapatite (HAP) composite were investigated. The fibrous composite of PVA/HAP/folic acid (FA)/Methotrexate (MTX) was coated on the surface of a titanium plate and observed with microscopy. During the PVA/HAP/FA/MTX composite's preparation, the functional changes and crystalline phases were observed through Fourier-transform infrared spectroscopy and X-ray diffraction analysis, respectively. The fiber composite had an average diameter of 19 nm and a greater mechanical strength (9660 Pa) than that of pure HAP (4965 Pa), critical to the fibrous scaffold under load-bearing applications. Biocompatibility, cell growth, and proliferation capability of the PVA/HAP/FA/MTX composite were studied in human bone marrow-derived stem cells (hBMSCs). The fiber composite was found to have excellent biocompatibility with significant cell growth, while the MTX-loaded PVA/HAP/FA composite showed cytotoxicity against A459 cells. The prepared fibrous composites will serve as a superior biomaterial for osteosarcoma-diseased bone repair after evidencing of in-vivo and clinical trials.

研究了用聚乙烯醇 (PVA) 增强羟基磷灰石 (HAP) 复合材料涂覆的钛 (Ti) 金属植入物的成骨细胞生长和癌细胞抑制作用。将 PVA/HAP/叶酸 (FA)/甲氨蝶呤 (MTX) 的纤维复合物涂覆在钛板表面并用显微镜观察。在PVA/HAP/FA/MTX复合材料的制备过程中，分别通过傅里叶变换红外光谱和X射线衍射分析观察了其功能变化和晶相。纤维复合材料的平均直径为 19 nm，机械强度 (9660 Pa) 比纯 HAP (4965 Pa) 更高，这对承重应用下的纤维支架至关重要。生物相容性、细胞生长、研究了 PVA/HAP/FA/MTX 复合材料在人骨髓源性干细胞 (hBMSCs) 中的增殖能力和增殖能力。发现纤维复合材料具有优异的生物相容性和显着的细胞生长，而负载 MTX 的 PVA/HAP/FA 复合材料对 A459 细胞显示出细胞毒性。所制备的纤维复合材料将作为一种优良的生物材料用于骨肉瘤病骨修复。体内试验和临床试验。