Abstract

A phytochemical investigation on the 80% ethanol extract of the roots of Caragana stenophylla Pojark. resulted in the isolation of 20 compounds, including two new ones, named kompasinol P (2) and 3,5,7,2',3'-pentahydroxy-4'-methoxyisoflavanone (3). Among them, a pair of enantiomers, (7S, 8 R, 7′R, 8′S)-kompasinol A (1a) and (7 R, 8S, 7′S, 8′R)-kompasinol A (1b), were successfully separated by the chiral-phase HPLC resolution for the first time. The absolute configurations of 1a and 1b were determined by the experimental and calculated electronic circular dichroism (ECD) data. 15 isolates were evaluated for their anti-inflammatory activity via inhibiting the production of nitric oxide (NO) in lipopolysaccharide (LPS)-stimulated murine macrophage RAW 264.7 cells. Compounds 1a/1b, 6, 7, 9, 10, 12, 14, and 16–18 showed moderate inhibition with IC₅₀ values ranging from 11.45 to 68.54 μM.

摘要

狭叶锦鸡儿根的 80% 乙醇提取物的植物化学研究。结果分离出 20 种化合物，包括两种新化合物，分别命名为 kompasinol P ( 2 ) 和 3,5,7,2',3'-五羟基-4'-甲氧基异黄烷酮 ( 3 )。其中，一对对映异构体，（7小号，8  - [R，7' - [R，8'小号）- kompasinol A（1A）和（7  - [R，8小号，7'小号，8' - [R）-kompasinol A（1B )，首次通过手性相 HPLC 拆分成功分离。绝对配置图 1a和1b是由实验和计算的电子圆二色性 (ECD) 数据确定的。通过抑制脂多糖 (LPS) 刺激的鼠巨噬细胞 RAW 264.7 细胞中一氧化氮 (NO) 的产生，评估了 15 个分离株的抗炎活性。化合物1A / 1B，6，7，9，10，12，14，和16 - 18显示中度抑制与IC _50个值范围从11.45至68.54微米。