Abstract

Photon detectors are important elements of optical analytical systems, such as electrochemiluminescence (ECL), and are used to determine the signal intensity. To accurately receive the photon signal in ECL, the detector should cover the wavelength range from approximately 400 to 700 nm for ECL experiments. However, the signal intensity is often too weak and cannot be observed by the naked eye or quantified digitally. Therefore, it is important to align the detector to the surface of the reaction site for the ECL system. In this minireview, a summary and comparison of photon-collecting tools to facilitate further study for choosing the proper detector of each ECL system is provided. Among all devices, charge couple device (CCD) cameras have gained significant attention in recent years. A CCD-integrated ECL device helps capture instant images and possess benefits such as miniaturization, portability, and low cost, which is in contrast to the alternatives. CCDs are utilized for diverse applications, including single-cell imaging, detection of molecules in cells and real samples, and the development of point-of-care testing, and are highly specific and provide ultra-sensitive detection. The signal collection is the last step in developing an ideal ECL system. The superior selectivity and sensitivity for the target of interest is promising for the construction of highly effective ECL biosensors.

摘要

光子探测器是光学分析系统的重要元素，例如电化学发光 (ECL)，用于确定信号强度。为了准确接收 ECL 中的光子信号，检测器应覆盖大约 400 至 700 nm 的波长范围以进行 ECL 实验。然而，信号强度通常太弱，无法用肉眼观察或数字量化。因此，将检测器对准 ECL 系统的反应部位表面非常重要。在这篇小评论中，提供了对光子收集工具的总结和比较，以促进进一步研究为每个 ECL 系统选择合适的探测器。在所有设备中，电荷耦合器件（CCD）相机近年来受到了极大的关注。集成 CCD 的 ECL 设备有助于捕获即时图像，并具有小型化、便携性和低成本等优势，这与其他替代产品形成鲜明对比。CCD 可用于多种应用，包括单细胞成像、细胞和真实样本中分子的检测以及即时检测的开发，并且具有高度特异性并提供超灵敏检测。信号采集是开发理想 ECL 系统的最后一步。对感兴趣的目标具有卓越的选择性和灵敏度，有望用于构建高效的 ECL 生物传感器。和即时检测的发展，具有高度特异性并提供超灵敏检测。信号采集是开发理想 ECL 系统的最后一步。对感兴趣的目标具有卓越的选择性和灵敏度，有望用于构建高效的 ECL 生物传感器。和即时检测的发展，具有高度特异性并提供超灵敏检测。信号采集是开发理想 ECL 系统的最后一步。对感兴趣的目标具有卓越的选择性和灵敏度，有望用于构建高效的 ECL 生物传感器。