Artemisia splendens from the Asteraceae family is a new source of biologically active compounds. The current study investigated to evaluate antimicrobial and cytotoxicity activity of methanolic extracts and their fractions obtained from aerial parts by agar disk diffusion and MTT methods, respectively. The active fractions were subjected to preparative HPLC for isolating the pure compounds, which were structurally elucidated, by 1H and 13C NMR. The results showed that the methanolic extract and its 60% SPE fraction have the anti-proliferative activity on A549 cell line in comparison with the control group. Meanwhile, the methanolic extract and its 40% SPE fraction can inhibit the growth of Gram-positive strains as anti-microbial activity. The 60% SPE fraction also illustrated anti-proliferative activity on the HT-29 cell line compared to the control group. Chromatographic separations via preparative HPLC yielded 5 flavonoids and three flavonoid glycosides. Based on the results it can be concluded that A. splendens as a potential source of cytotoxic and antimicrobial compounds can be used in pharmaceutics.

中文翻译：

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在伊朗生长的亚洲小艾草（Artemisia splendens Willd 。）的化学成分，抗菌作用和细胞毒性

菊科的蒿（Artemisia splendens）是生物活性化合物的新来源。目前的研究旨在评估琼脂圆盘扩散法和MTT法从地上部分获得的甲醇提取物及其馏分的抗微生物和细胞毒性活性。将活性级分进行制备型HPLC以分离通过1 H和13 C NMR在结构上阐明的纯化合物。结果表明，与对照组相比，甲醇提取物及其60％SPE组分对A549细胞具有抗增殖活性。同时，甲醇提取物及其40％SPE组分可抑制革兰氏阳性菌株的生长，具有抗微生物活性。与对照组相比，60％SPE馏分还显示了对HT-29细胞系的抗增殖活性。通过制备型HPLC进行色谱分离，得到5个黄酮类化合物和3个黄酮类苷。基于该结果，可以得出结论，作为一种潜在的细胞毒性和抗菌化合物的来源，sp草曲霉可以用于药物中。