The use of antibiotics in animal agriculture has exacerbated the presence of both antibiotic resistance genes (ARGs) and residual antibiotics excreted in animal manure. Field application of this manure is a common practice because its nutrient rich material can benefit crop growth. However, this practice can also introduce antibiotics and ARGs into nonagricultural settings. The integration of prairie buffer strips within and at the edge of crop fields is a potential management solution to reduce concentrations of ARGs commonly transported via water runoff and infiltration. An incubation experiment was conducted to investigate the fate of ARGs in directly manured crop field soils and the surrounding affected prairie strip soils. Row crop and prairie strip soils sampled from three sites received either an antibiotic spike and swine manure addition or a control water addition. The concentrations of select ARGs were then monitored over a 72-d period. Although soil vegetation and site location were not observed to influence ARG dissipation, the select genes did display different half-lives from one another. For example, tetM demonstrated the fastest dissipation of the genes quantified (average half-life, 5.18 d). Conversely, sul1 did not conform to the first-order linear regression kinetics used to describe the other investigated genes and was highly abundant in control prairie strip soils. The quantified half-lives of these select ARGs are comparable to previous studies and can inform monitoring and mitigative efforts aimed at reducing the spread of ARGs in the environment.

中文翻译：

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用抗生素和猪粪修正土壤微观世界中抗生素抗性基因的耗散

在畜牧业中使用抗生素加剧了抗生素抗性基因 (ARG) 和动物粪便中残留抗生素的存在。这种肥料的田间施用是一种常见做法，因为其营养丰富的材料可以有益于作物生长。然而，这种做法也可能将抗生素和 ARGs 引入非农业环境。在农田内部和边缘整合草原缓冲带是一种潜在的管理解决方案，可降低通常通过水径流和渗透传输的 ARGs 浓度。进行孵化实验以研究 ARGs 在直接施肥的农田土壤和周围受影响的草原地带土壤中的归宿。从三个地点取样的中耕作物和草原地带土壤接受了抗生素穗和猪粪添加或控制水添加。然后在 72 天的时间内监测所选 ARG 的浓度。尽管未观察到土壤植被和场地位置影响 ARG 耗散，但所选基因确实显示出彼此不同的半衰期。例如，tetM显示量化基因的最快消散（平均半衰期，5.18 天）。相反，sul1不符合用于描述其他研究基因的一阶线性回归动力学，并且在对照草原带土壤中高度丰富。这些选定 ARG 的量化半衰期与之前的研究相当，可以为旨在减少 ARG 在环境中传播的监测和缓解工作提供信息。