Introduction:

SKF-96365 is regarded as an inhibitor of receptor-mediated calcium ion (Ca²⁺) entry. The current study aimed to explore the effects of SKF-96365 on murine allergic rhinitis (AR).

Methods:

Intranasal SKF-96365 administration was performed on OVA induced murine AR. Serum and nasal lavage fluid (NLF) from mice were harvested to assay IgE and inflammatory cytokines using ELISA method. Inflammatory cells were counted and analyzed in NLF. Nasal mucosa tissues were collected from mice and used for HE staining, immunohistochemistry (IHC) staining, and real-time PCR detection.

Results:

SKF-96365 had therapeutic effects on murine AR manifesting attenuation of sneezing, nasal rubbing, IgE, inflammatory cytokines, inflammatory cells, TRPC6 immunolabeling, and TRPC6, STIM1 and Orai1 mRNA levels in AR mice.

Conclusion:

SKF-96365 could effectively alleviate the symptoms of murine AR. SKF-96365 could suppress TRPC6, STIM1, and Orai1 activities, leading to the downregulation of inflammatory cytokines and inflammatory cells in murine AR.

中文翻译：

---

SKF-96365对OVA所致小鼠过敏性鼻炎的治疗作用

 介绍：

SKF-96365 被认为是受体介导的钙离子 (Ca ²⁺ ) 进入的抑制剂。目前的研究旨在探讨 SKF-96365 对小鼠过敏性鼻炎 (AR) 的影响。

 方法：

对 OVA 诱导的小鼠 AR 进行鼻内 SKF-96365 给药。收集小鼠血清和鼻腔灌洗液 (NLF)，使用 ELISA 方法测定 IgE 和炎症细胞因子。在 NLF 中对炎症细胞进行计数和分析。采集小鼠鼻粘膜组织，用于HE染色、免疫组织化学（IHC）染色和实时PCR检测。

 结果：

SKF-96365 对小鼠 AR 具有治疗作用，表现为 AR 小鼠中打喷嚏、擦鼻、IgE、炎症细胞因子、炎症细胞、TRPC6 免疫标记以及 TRPC6、STIM1 和 Orai1 mRNA 水平减弱。

 结论：

SKF-96365可以有效缓解小鼠AR症状。 SKF-96365 可以抑制 TRPC6、STIM1 和 Orai1 活性，导致小鼠 AR 中炎症细胞因子和炎症细胞的下调。