In Arabidopsis thaliana (L.) Heynh., MYB59 transcription factor participates in regulating Ca homeostasis and signal transduction and is induced by Cd excess. To investigate its role in the facultative metallophyte Arabidopsis halleri ssp. halleri (L.) O’Kane and Al-Shehbaz, MYB59 expression was investigated under Cd treatment or Ca depletion in three populations belonging to distinct phylogeographic units (metallicolous PL22 and I16 and non-metallicolous I29), and compared with the expression in A. thaliana. In control conditions, MYB59 transcription in A. thaliana and the non-metallicolous population I29 follow a comparable trend with higher expression in roots than shoots, whereas in metallicolous populations I16 and PL22 its expression is similar in roots and shoots, suggesting a convergent evolution associated with adaptation to metalliferous environments. After 6 h of Ca depletion, MYB59 transcript levels were very high in I16 and PL22 populations, indicating that the adaptation to metalliferous environments requires tightly regulated Ca homeostasis and signalling. Cd treatment caused variability in MYB59 expression. In I29, MYB59 expression, as in A. thaliana, is likely associated to stress response, whereas its modulation in the two metallicolous populations reflects the different strategies for Cd tolerance and accumulation. In conclusion, MYB59 regulation in A. halleri is part of the network linking mineral nutrition and Cd tolerance/accumulation.

在拟南芥(L.) Heynh. 中，MYB59 转录因子参与调节 Ca 稳态和信号转导，并由 Cd 过量诱导。研究其在兼性金属植物拟南芥中的作用。Halleri (L.) O'Kane 和 Al-Shehbaz，在属于不同系统地理单元（金属 PL22 和 I16 和非金属 I29）的三个种群中，在 Cd 处理或 Ca 消耗下研究了MYB59的表达，并与 A 中的表达进行了比较.拟南芥 在对照条件下，拟南芥中的MYB59转录和非金属种群 I29 遵循类似的趋势，根中的表达高于芽，而在金属种群 I16 和 PL22 中，其在根和芽中的表达相似，表明与金属环境适应相关的趋同进化。Ca 耗尽 6 小时后，MYB59转录水平在 I16 和 PL22 种群中非常高，表明对金属环境的适应需要严格调节 Ca 稳态和信号传导。Cd 处理导致MYB59表达的变异性。在 I29 中，MYB59表达，如在拟南芥中，可能与应激反应有关，而其在两种金属群中的调节反映了对镉耐受和积累的不同策略。总之，A.halleri中的MYB59调节是连接矿物质营养和 Cd 耐受/积累网络的一部分。