Significance: Confocal laser scanning enables optical sectioning in fiber bundle endomicroscopy but limits the frame rate. To be able to better explore tissue morphology, it is useful to stitch sequentially acquired frames into a mosaic. However, low frame rates limit the maximum probe translation speed. Line-scanning (LS) confocal endomicroscopy provides higher frame rates, but residual out-of-focus light degrades images. Subtraction-based approaches can suppress this residue at the expense of introducing motion artifacts. Aim: To generate high-frame-rate endomicroscopy images with improved optical sectioning, we develop a high-speed subtraction method that only requires the acquisition of a single camera frame. Approach: The rolling shutter of a CMOS camera acts as both the aligned and offset detector slits required for subtraction-based sectioning enhancement. Two images of the bundle are formed on different regions of the camera, allowing both images to be acquired simultaneously. Results: We confirm improved optical sectioning compared to conventional LS, particularly far from focus, and show that motion artifacts are not introduced. We demonstrate high-speed mosaicing at frame rates of up to 240 Hz. Conclusion: High-speed acquisition of optically sectioned images using the new subtraction based-approach leads to improved mosaicing at high frame rates.

中文翻译：

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在增强型线扫描光纤束内镜检查中减少运动伪影并提高速度

意义：共聚焦激光扫描能够在纤维束内镜下进行光学切片，但会限制帧速率。为了能够更好地探索组织形态，将顺序获取的帧拼接成马赛克很有用。然而，低帧率限制了最大探针转换速度。线扫描 (LS) 共聚焦内窥镜提供更高的帧速率，但残留的失焦光会降低图像质量。基于减法的方法可以以引入运动伪影为代价来抑制这种残留。目的：为了生成具有改进光学切片的高帧率内窥镜图像，我们开发了一种仅需要获取单个相机帧的高速减法方法。方法：CMOS 相机的滚动快门充当基于减法的切片增强所需的对齐和偏移检测器狭缝。束的两个图像形成在相机的不同区域，允许同时获取两个图像。结果：我们确认与传统 LS 相比改进了光学切片，特别是远离焦点，并表明没有引入运动伪影。我们演示了高达 240 Hz 的帧速率的高速拼接。结论：使用基于减法的新方法高速采集光学切片图像可改善高帧速率下的拼接。我们确认与传统 LS 相比改进了光学切片，特别是远离焦点，并表明没有引入运动伪影。我们演示了高达 240 Hz 的帧速率的高速拼接。结论：使用基于减法的新方法高速采集光学切片图像可改善高帧速率下的拼接。我们确认与传统 LS 相比改进了光学切片，特别是远离焦点，并表明没有引入运动伪影。我们演示了高达 240 Hz 的帧速率的高速拼接。结论：使用基于减法的新方法高速采集光学切片图像可改善高帧速率下的拼接。