Microalgae, a kind of conventional bait for bivalves, can increase the cost of shellfish aquaculture. On this basis, Ruditapes philippinarum spat was selected to evaluate the feasibility of using photosynthetic bacteria (PSB) and marine yeasts as microalgae substitutes. The results showed that the addition of bacteria obviously affected the growth status of spat. Firstly, the survival rate of spat in 5%, 40%, 50% and 70% PSB substitution groups as well as 5% and 40% yeast substitution groups was not significantly different from that in microalgae group (p > 0.05), but that in other substitution groups was significantly lower than that in microalgae group (p < 0.05). Secondly, the growth rate of spat in PSB substitution groups (except for the 10% PSB substitution group) was significantly higher than that in microalgae group, and the 5% PSB substitution group had a growth rate which was 109.55% higher than that in microalgae group. There was no significant difference in the growth rate between microalgae group and 10%, 40%, 50% and 70% yeast substitution groups. Thirdly, the addition of bacteria also affected the digestive ability of shellfish. Pepsin enzyme activity in 20% PSB substitution group was 29.85% higher than that in microalgae group. Moreover, 5% and 40% yeast substitution groups had no significant difference in the pepsin enzyme activity compared with microalgae group. Finally, the nitrogen (N) and phosphorus (P) concentrations in the bacteria addition treatments were higher than those in the control treatment at the end of the experiment, but they were generally maintained at lower levels. In conclusion, it is feasible to partially replace microalgae with PSB and marine yeasts, and it is suggested that shellfish culture should be combined with wastewater utilization to promote the healthy development of aquaculture industry.

中文翻译：

---

两种益生菌替代微藻替代对虾养殖废水养殖菲律宾蛤仔的可行性评价

微藻是一种双壳类常规饵料，会增加贝类养殖成本。在此基础上，选择菲律宾蛤仔卵来评价利用光合细菌（PSB）和海洋酵母作为微藻替代品的可行性。结果表明，细菌的加入对卵的生长状态有明显影响。首先，5%、40%、50%和70% PSB替代组以及5%和40%酵母替代组的幼苗存活率与微藻组无显着差异（p  > 0.05），但其他替代组显着低于微藻组（p < 0.05)。其次，PSB替代组（10%PSB替代组除外）的卵子生长速率显着高于微藻组，5%PSB替代组的生长速率比微藻组高109.55%团体。微藻组与10%、40%、50%和70%酵母替代组的生长速率无显着差异。第三，细菌的加入也影响了贝类的消化能力。20%PSB替代组胃蛋白酶酶活性比微藻组高29.85%。此外，与微藻组相比，5%和40%酵母替代组的胃蛋白酶活性没有显着差异。最后，实验结束时细菌添加处理的氮（N）和磷（P）浓度高于对照处理，但总体保持在较低水平。综上所述，用PSB和海洋酵母部分替代微藻是可行的，建议贝类养殖与废水利用相结合，促进水产养殖业的健康发展。