The growth, development, and quality production of Rosa hybrida L. are the core issues for being able to have good yields. Inadequate information on genetic background and resistance has hindered the ability to continue improving flower quality, and most cultivars continue to have lower-than-desired levels of resistance. Therefore, this study sequenced the transcription group of the R. chinensis Jacq. ‘Old Blush’ under high salt stress. Transcription group sequencing was carried out on root and leaf materials at different points in time (0, 2, 24, and 48 h) under a high salt stress condition of NaHCO₃. The monthly salt stress recorded within 48-h Ca²⁺ sensors was mainly calmodulin and calcium-binding protein. The kinase 5 (RC2G0184500) began to show significant increase from 24- and 48-h process time, respectively, within the leaf blades and the root. There was an increase of superoxide dismutase and L-ascorbate peroxidase from the 2- and 48-h processing times, respectively. Within the blades, MYB4, MYB41, MYB44, MYB20, MYB62, and MYB14 had a log2Foldchange of >4. ERF109 had a log2Foldchange of 7. NAC-like transcription factors had more differential genes in the root than in the leaves, with a log2Foldchange of NAC67, NAC52, NAC16, and NAC2 of >4. The plant hormone signal transduction-related genes involved were abscisic acid, growth hormone, ethylene, erythromycin, jasmine acid, salbonate, and cell division. The EIN4 and EIN3 were only raised in the leaf and the serine/threonine-protein kinase CTR1 was raised in the root, whereas the ethylene pathway was mainly in the leaves. Auxin-responsive protein SAUR72 and SAUR50 were raised in the leaves and the other auxin-responsive protein and SAUR expression in the root were low. The IAA2 was revised upward in the leaf and the IAA11 was revised upward in the root, whereas the rest were revised downward. In conclusion, the study revealed several genes and hormones that are key in salt stress response.

中文翻译：

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蔷薇“老红”的比较转录组分析提供了对盐胁迫响应的关键因素和信号通路的见解

Rosa hybrida L.的生长、发育和优质生产是能够获得良好产量的核心问题。遗传背景和抗性方面的信息不足阻碍了继续提高花卉质量的能力，大多数品种的抗性水平仍然低于预期水平。因此，本研究对R. chinensis Jacq的转录组进行了测序。高盐胁迫下的“老脸红”。在NaHCO ₃高盐胁迫条件下，在不同时间点（0、2、24和48 h）对根和叶材料进行转录组测序。48 小时 Ca ²⁺内记录的每月盐胁迫传感器主要是钙调蛋白和钙结合蛋白。在叶片和根部，激酶 5 (RC2G0184500) 开始分别从 24 小时和 48 小时处理时间显着增加。超氧化物歧化酶和 L-抗坏血酸过氧化物酶分别从 2 小时和 48 小时处理时间增加。在叶片中，MYB4、MYB41、MYB44、MYB20、MYB62 和 MYB14 的 log2Foldchange 大于 4。ERF109 的 log2Foldchange 为 7。NAC 样转录因子在根中的差异基因多于叶子，NAC67、NAC52、NAC16 和 NAC2 的 log2Foldchange 大于 4。涉及的植物激素信号转导相关基因有脱落酸、生长激素、乙烯、红霉素、茉莉酸、盐酸盐、细胞分裂等。EIN4 和 EIN3 仅在叶片中升高，丝氨酸/苏氨酸蛋白激酶 CTR1 在根中升高，而乙烯途径主要在叶片中升高。叶片中生长素响应蛋白 SAUR72 和 SAUR50 升高，根中其他生长素响应蛋白和 SAUR 表达较低。IAA2 在叶中向上修正，IAA11 在根中向上修正，而其余部分向下修正。总之，该研究揭示了几种在盐胁迫反应中起关键作用的基因和激素。IAA2 在叶中向上修正，IAA11 在根中向上修正，而其余部分向下修正。总之，该研究揭示了几种在盐胁迫反应中起关键作用的基因和激素。IAA2 在叶中向上修正，IAA11 在根中向上修正，而其余部分向下修正。总之，该研究揭示了几种在盐胁迫反应中起关键作用的基因和激素。