Two Bemisia tabaci (Gennadius) species, Middle East-Asia Minor 1 (MEAM1) and Mediterranean (MED), are major pests that are dispersed throughout the world. While MEAM1 was introduced in Brazil in the 1990s, MED was reported recently with limited spread. Here, a survey was performed to examine whether MED whiteflies are widely present in the Federal District region, in central Brazil. Whiteflies were collected in various locations in the Federal District and surroundings between 2018 and 2020, including garden centers and small- and large-scale farms. The species were identified using RFLPand sequencing of the mitochondrial cytochrome c oxidase I subunit gene region. Out of 108 whitefly batches, 63.89% were composed exclusively by MEAM1, followed by 16.67% presenting only MED, and another 7.40% containing unidentified whitefly species (NI). Plant varieties serving as hosts for more than one whitefly species were observed in 12.04% of the samples, either by MEAM1/MED, MEAM1/NI, or MED/NI. This study highlights the still limited presence of MED in the Federal District and surroundings, predominantly in garden centers and in the green belt of Brasília, closer to urban areas. In contrast, only MEAM1 was identified in large-scale cultivated areas.

两种烟粉虱(Gennadius) 物种，中东-小亚细亚 1 (MEAM1) 和地中海 (MED)，是分布在世界各地的主要害虫。虽然 MEAM1 于 1990 年代在巴西被引入，但最近报道的 MED 传播有限。在这里，进行了一项调查，以检查 MED 粉虱是否广泛存在于巴西中部的联邦区。2018 年至 2020 年期间，在联邦区和周边地区的不同地点收集了粉虱，包括花园中心和小型和大型农场。使用RFLP和线粒体细胞色素c氧化酶I测序鉴定物种亚单位基因区。在 108 个粉虱批次中，63.89% 完全由 MEAM1 组成，其次是 16.67% 仅呈现 MED，另外 7.40% 包含不明粉虱物种 (NI)。在 12.04% 的样本中，通过 MEAM1/MED、MEAM1/NI 或 MED/NI 观察到作为一种以上粉虱物种宿主的植物品种。这项研究强调了 MED 在联邦区和周边地区的存在仍然有限，主要是在花园中心和巴西利亚的绿化带中，靠近城市地区。相比之下，在大规模耕作区仅鉴定了 MEAM1。