Glycan microarrays have played important roles in detection and specificity assignment of glycan recognition by proteins. However, the size and diversity of glycan libraries in current microarray systems are small compared to estimated glycomes, and these may lead to missed detection or incomplete assignment. For microarray construction, covalent and noncovalent immobilization are the two types of methods used, but a direct comparison of results from the two platforms is required. Here we develop a chemical strategy to prepare lipid-linked probes from both naturally derived aldehyde-terminating and synthetic amino-terminating glycans that addresses the two aspects: expansion of sequence-defined glycan libraries and comparison of the two platforms. We demonstrate the specific recognition by plant and mammalian lectins, carbohydrate-binding modules and antibodies and the overall similarities from the two platforms. Our results provide new knowledge on unique glycan-binding specificities for the immune receptor Dectin-1 toward β-glucans and the interaction of rotavirus P[19] adhesive protein with mucin O-glycan cores.

中文翻译：

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来自合成氨基末端聚糖的非共价微阵列：扩展聚糖微阵列多样性和平台比较的意义

聚糖微阵列在蛋白质识别聚糖的检测和特异性分配中发挥了重要作用。然而，与估计的糖组相比，当前微阵列系统中聚糖库的大小和多样性都很小，这可能导致漏检或分配不完整。对于微阵列构建，共价和非共价固定是使用的两种方法，但需要直接比较两种平台的结果。在这里，我们开发了一种化学策略，从天然衍生的醛终止和合成氨基终止聚糖制备脂质连接探针，解决两个方面：序列定义聚糖库的扩展和两个平台的比较。我们证明了植物和哺乳动物凝集素的特异性识别，碳水化合物结合模块和抗体以及两个平台的总体相似性。我们的研究结果为免疫受体 Dectin-1 对 β-葡聚糖的独特聚糖结合特异性以及轮状病毒 P[19] 粘附蛋白与粘蛋白的相互作用提供了新知识O-聚糖核心。