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Influence of Hydrogen Bond and Sodium Alginate on Bovine Serum Albumin Adhesion on ZnSe Surface
Chinese Journal of Analytical Chemistry ( IF 1.2 ) Pub Date : 2021-05-12 , DOI: 10.1016/s1872-2040(21)60101-9
Xiao-Xuan ZHANG , Wu-Bo CHU , Nan JIANG , He LI

Serum albumin is the most abundant protein in mammalian plasma and is often used as a model to understand the adhesion behavior of proteins on surfaces. Attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) is used to non-destructively and dynamically detect the chemical change information between the protein and the surface during the adhesion process, which is especially suitable for in-situ measurement of samples in water. Therefore, the adhesion of bovine serum albumin (BSA) on the surface in H2O and D2O is investigated by ATR-FTIR technology, which effectively avoids the interference of the characteristic peak of deformation vibration of liquid H2O (1645 cm−1) on the amide I band of BSA. Sodium alginate, a polysaccharide widely present in the ocean, is introduced into the BSA solution to explore the changes in the adhesion process of proteins on the surface of ZnSe, and polarized ATR-FTIR spectra are also collected to study the adhesion orientation of BSA. Sodium alginate presents a negative effect on the adhesion of BSA on the surface and there is no specific orientation of BSA on the surface.



中文翻译:

氢键和海藻酸钠对牛血清白蛋白在ZnSe表面粘附的影响

血清白蛋白是哺乳动物血浆中最丰富的蛋白质,通常用作了解蛋白质在表面上的粘附行为的模型。衰减全反射傅里叶变换红外光谱(ATR-FTIR)用于无损,动态地检测粘附过程中蛋白质与表面之间的化学变化信息,特别适用于原位测量水中的样品。因此,采用ATR-FTIR技术研究了牛血清白蛋白(BSA)在H 2 O和D 2 O表面的粘附性,有效避免了液体H 2 O(1645 cm -1)在BSA的酰胺I谱带上。将海藻酸钠(一种广泛存在于海洋中的多糖)引入BSA溶液中,以研究蛋白质在ZnSe表面的粘附过程的变化,并收集极化的ATR-FTIR光谱以研究BSA的粘附方向。海藻酸钠对BSA在表面上的粘附具有负面影响,并且在表面上没有BSA的特定取向。

更新日期:2021-05-12
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