Serum albumin is the most abundant protein in mammalian plasma and is often used as a model to understand the adhesion behavior of proteins on surfaces. Attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) is used to non-destructively and dynamically detect the chemical change information between the protein and the surface during the adhesion process, which is especially suitable for in-situ measurement of samples in water. Therefore, the adhesion of bovine serum albumin (BSA) on the surface in H₂O and D₂O is investigated by ATR-FTIR technology, which effectively avoids the interference of the characteristic peak of deformation vibration of liquid H₂O (1645 cm⁻¹) on the amide I band of BSA. Sodium alginate, a polysaccharide widely present in the ocean, is introduced into the BSA solution to explore the changes in the adhesion process of proteins on the surface of ZnSe, and polarized ATR-FTIR spectra are also collected to study the adhesion orientation of BSA. Sodium alginate presents a negative effect on the adhesion of BSA on the surface and there is no specific orientation of BSA on the surface.

血清白蛋白是哺乳动物血浆中最丰富的蛋白质，通常用作了解蛋白质在表面上的粘附行为的模型。衰减全反射傅里叶变换红外光谱（ATR-FTIR）用于无损，动态地检测粘附过程中蛋白质与表面之间的化学变化信息，特别适用于原位测量水中的样品。因此，采用ATR-FTIR技术研究了牛血清白蛋白（BSA）在H ₂ O和D ₂ O表面的粘附性，有效避免了液体H ₂ O（1645 cm ^-1）在BSA的酰胺I谱带上。将海藻酸钠（一种广泛存在于海洋中的多糖）引入BSA溶液中，以研究蛋白质在ZnSe表面的粘附过程的变化，并收集极化的ATR-FTIR光谱以研究BSA的粘附方向。海藻酸钠对BSA在表面上的粘附具有负面影响，并且在表面上没有BSA的特定取向。