Hepatocellular carcinoma (HCC) is a highly malignant tumor. In this study, we sought to identify a novel biomarker for HCC by analyzing transcriptome and clinical data. The R software was used to analyze the differentially expressed genes (DEGs) in the datasets GSE74656 and GSE84598 downloaded from the Gene Expression Omnibus database, followed by a functional annotation. A total of 138 shared DEGs were screened from two datasets. They were mainly enriched in the "Metabolic pathways" pathway (Padj = 8.21E-08) and involved in the carboxylic acid metabolic process (Padj = 0.0004). The top 10 hub genes were found by protein-protein interaction analysis and were upregulated in HCC tissues compared to normal tissues in The Cancer Genome Atlas database. Survival analysis distinguished 8 hub genes CENPE, SPDL1, Hyaluronan-mediated motility receptor, Rac GTPase activating protein 1, Thyroid hormone receptor interactor 13, cytoskeleton-associated protein (CKAP) 2, CKAP5, and Integrin subunit beta 3 binding protein (ITGB3BP) were considered as prognostic hub genes. Multivariate cox regression analysis indicated that all the prognostic hub genes were independent prognostic factors for HCC. Furthermore, the receiver operating characteristic curve revealed that the 8-hub genes model had better prediction performance for overall survival compared to the T stage (p = 0.008) and significantly improved the prediction value of the T stage (p = 0.002). The Human Protein Atlas showed that the protein expression of ITGB3BP was upregulated in HCC, so the expression of ITGB3BP was further verified in our cohort. The results showed that ITGB3BP was upregulated in HCC tissues and was significantly associated with lymph node metastasis.

中文翻译：

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综合分析将 ITGB3BP 确定为肝细胞癌的预后生物标志物。

肝细胞癌（HCC）是一种高度恶性的肿瘤。在这项研究中，我们试图通过分析转录组和临床数据来确定一种新的 HCC 生物标志物。R软件用于分析从Gene Expression Omnibus数据库下载的数据集GSE74656和GSE84598中的差异表达基因（DEG），然后进行功能注释。从两个数据集中筛选出总共 138 个共享的 DEG。它们主要富集在“代谢途径”途径（Padj = 8.21E-08）并参与羧酸代谢过程（Padj = 0.0004）。前 10 个中心基因是通过蛋白质-蛋白质相互作用分析发现的，并且与癌症基因组图谱数据库中的正常组织相比，在 HCC 组织中上调。生存分析区分了 8 个中心基因 CENPE、SPDL1、透明质酸介导的运动受体、Rac GTPase 激活蛋白 1、甲状腺激素受体相互作用物 13、细胞骨架相关蛋白 (CKAP) 2、CKAP5 和整合素亚基 β 3 结合蛋白 (ITGB3BP) 被认为是预后中枢基因。多变量cox回归分析表明，所有的预后hub基因都是HCC的独立预后因素。此外，受试者工作特征曲线显示，8-hub 基因模型对总生存期的预测性能优于 T 期（p = 0.008），并显着提高了 T 期的预测值（p = 0.002）。人类蛋白质图谱显示ITGB3BP的蛋白质表达在HCC中上调，因此ITGB3BP的表达在我们的队列中得到进一步验证。