More and more evidence shows that microRNAs (miRNAs) play an important role in the diagnosis and prognosis of human diseases. In this study, we investigated the diagnostic value of miR-9-5p for asymptomatic carotid artery stenosis (CAS) and its predictive value for future cerebrovascular events within 5 years. A total of 88 asymptomatic CAS patients and 86 healthy individuals were recruited. The expression level of serum miR-9-5p was determined by quantitative real-time polymerase chain reaction (qRT-PCR). The diagnostic value of miR-9-5p in CAS was assessed by a receiving operator characteristic (ROC) curve. The predictive value of miR-9-5p for the occurrence of cerebrovascular events was evaluated by the Kaplan-Meier method. The serum level of miR-9-5p was significantly decreased in asymptomatic CAS patients. ROC curve had an AUC value of 0.910, with the sensitivity and specificity of 80.7% and 87.2% at the cut-off value of 0.72, respectively. A total of 25 patients had cerebrovascular events during the 5-year follow-up, including 3 strokes and 22 transient ischemic attacks (TIAs). Kaplan-Meier survival analysis revealed that the low expression level of miR-9-5p was an independent factor closely related to the occurrence of cerebrovascular events. Serum miR-9-5p could be used as a new biomarker for the diagnosis of CAS, and the low expression of miR-9-5p is associated with poor prognosis.

中文翻译：

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miR-9-5p在颈动脉狭窄中的诊断和预后价值分析。

越来越多的证据表明，microRNAs（miRNAs）在人类疾病的诊断和预后中发挥着重要作用。在这项研究中，我们调查了 miR-9-5p 对无症状颈动脉狭窄 (CAS) 的诊断价值及其对 5 年内未来脑血管事件的预测价值。共招募了 88 名无症状 CAS 患者和 86 名健康人。通过定量实时聚合酶链反应（qRT-PCR）测定血清miR-9-5p的表达水平。通过接收操作者特征（ROC）曲线评估 miR-9-5p 在 CAS 中的诊断价值。Kaplan-Meier法评价miR-9-5p对脑血管事件发生的预测价值。无症状 CAS 患者的血清 miR-9-5p 水平显着降低。ROC 曲线的 AUC 值为 0.910，在临界值为 0.72 时，敏感性和特异性分别为 80.7% 和 87.2%。5 年随访期间共有 25 名患者发生脑血管事件，包括 3 次中风和 22 次短暂性脑缺血发作 (TIA)。Kaplan-Meier生存分析显示，miR-9-5p的低表达水平是与脑血管事件发生密切相关的独立因素。血清 miR-9-5p 可作为诊断 CAS 的新生物标志物，miR-9-5p 低表达与预后不良有关。Kaplan-Meier生存分析显示，miR-9-5p的低表达水平是与脑血管事件发生密切相关的独立因素。血清 miR-9-5p 可作为诊断 CAS 的新生物标志物，miR-9-5p 低表达与预后不良有关。Kaplan-Meier生存分析显示，miR-9-5p的低表达水平是与脑血管事件发生密切相关的独立因素。血清 miR-9-5p 可作为诊断 CAS 的新生物标志物，miR-9-5p 低表达与预后不良有关。