Meloidogyne incognita is a root-knot nematode that causes serious damage to crops. An actinomycete, Agromyces allii 130935, isolated from lichens exhibited nematicidal activity against second-stage juveniles of M. incognita. The nematicidal activity of the ethyl acetate phase of A. allii 130935 reached 89% and 96% at 8 h and 24 h, respectively. Four compounds, namely 2-hydroxyundecanoic acid, 4-(2-furanylcarbonyl)-3-hydroxy-5-phenyl-2(5H)-furanone, 1-(1H-pyrrol-2-yl)-methanediol, and 2-[[[(5-nitro-2-furanyl)carbonyl]amino]oxy]-propanoic acid, were isolated and identified from the ethyl acetate phase of A. allii 130935. All the four compounds showed a certain toxic effect against the second-stage juveniles of M. incognita at 24 h. Compound 2-[[[(5-nitro-2-furanyl)carbonyl]amino]oxy]-propanoic acid caused 50% mortality at a concentration of 200 μg mL⁻¹ within 24 h. Field experiments confirmed that the ethyl acetate extract of A. allii 130935 significantly reduced root galls, with a control effect 50%. Our results indicate that A. allii 130935 and its metabolites are potential biocontrol agents against M. incognita.

根结线虫（Meloidogyne incognita）是一种根结线虫，会严重危害农作物。放线菌，Agromyces叶斑病130935，从地衣中分离表现出杀线虫活性针对第二阶段juveniles南方根结线虫。拟南芥130935的乙酸乙酯相的杀线虫活性分别在8小时和24小时达到89％和96％。四种化合物，即2-羟基十一烷酸，4-（2-呋喃基羰基）-3-羟基-5-苯基-2（5 H）-呋喃酮，1-（1 H-吡咯-2-基）-甲二醇和2从[ A. allii ]的乙酸乙酯相中分离并鉴定了-[[[[（5-硝基-2-呋喃基羰基）氨基]氧基]-丙酸。130935.所有这四种化合物在24 h时都对隐身梭状芽胞杆菌的第二阶段幼虫表现出一定的毒性作用。化合物2-[[[[（（5-硝基-2-呋喃基）羰基]氨基]氧基]-丙酸在24小时内以200μgmL ^-1的浓度引起50％的死亡率。野外实验证实，A。allii 130935的乙酸乙酯提取物可显着减少根gall，防治效果达50％。我们的研究结果表明A.叶斑病130935及其代谢产物对潜在生防菌南方根结线虫。