Glands of the uterus are essential for the establishment of pregnancy in mice and their products regulate embryo implantation and stromal cell decidualization critical for pregnancy establishment. Forkhead box A2 (FOXA2) is expressed specifically in the glands and a critical regulator of their differentiation, development and function. Progesterone and FOXA2 regulate members of a serine proteinase gene family (Prss28 and Prss29). Here, CRISPR-Cas9 genome-editing was used to create mice with a heterozygous or homozygous deletion of Prss28 or/and Prss29 to determine their biological roles in uterine function. Female mice lacking Prss28 and Prss29 or both developed normally and were fertile without alterations in uterine histoarchitecture, uterine gland number, or and gene expression. Thus, Prss28 and Prss29 are dispensable for female fertility and do not impact endometrial gland development or uterine function mice.

中文翻译：

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使用 CRISPR-Cas9 基因组编辑生成和分析 Prss28 和 Prss29 缺陷小鼠


子宫腺体对于小鼠妊娠的建立至关重要，其产物调节胚胎植入和基质细胞蜕膜化，这对于妊娠的建立至关重要。叉头盒 A2 (FOXA2) 在腺体中特异性表达，是腺体分化、发育和功能的关键调节因子。黄体酮和 FOXA2 调节丝氨酸蛋白酶基因家族的成员（ Prss28和Prss29） 。在这里，CRISPR-Cas9基因组编辑被用来创建具有Prss28或/和Prss29杂合或纯合缺失的小鼠，以确定它们在子宫功能中的生物学作用。缺乏Prss28和Prss29或两者都缺乏的雌性小鼠发育正常并且具有生育能力，子宫组织结构、子宫腺体数量或基因表达没有改变。因此，Prss28和Prss29对于雌性生育能力是可有可无的，并且不会影响子宫内膜腺体发育或子宫功能小鼠。