Applications of isothermal titration calorimetry in pure and applied research from 2016 to 2020,Journal of Molecular Recognition

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Applications of isothermal titration calorimetry in pure and applied research from 2016 to 2020
Journal of Molecular Recognition ( IF 2.3 ) Pub Date : 2021-05-11 , DOI: 10.1002/jmr.2901
Robert J Falconer ₁ , Boelo Schuur ₂ , Anthony K Mittermaier ₃

Affiliation

The last 5â€‰years have seen a series of advances in the application of isothermal titration microcalorimetry (ITC) and interpretation of ITC data. ITC has played an invaluable role in understanding multiprotein complex formation including proteolysis-targeting chimeras (PROTACS), and mitochondrial autophagy receptor Nix interaction with LC3 and GABARAP. It has also helped elucidate complex allosteric communication in protein complexes like trp RNA-binding attenuation protein (TRAP) complex. Advances in kinetics analysis have enabled the calculation of kinetic rate constants from pre-existing ITC data sets. Diverse strategies have also been developed to study enzyme kinetics and enzyme-inhibitor interactions. ITC has also been applied to study small molecule solvent and solute interactions involved in extraction, separation, and purification applications including liquid-liquid separation and extractive distillation. Diverse applications of ITC have been developed from the analysis of protein instability at different temperatures, determination of enzyme kinetics in suspensions of living cells to the adsorption of uremic toxins from aqueous streams.

中文翻译：

2016年至2020年等温滴定量热法在纯和应用研究中的应用

在过去的 5 年里，等温滴定微量热法 (ITC) 的应用和 ITC 数据的解释取得了一系列进展。ITC 在了解多蛋白复合物形成方面发挥了宝贵的作用，包括蛋白水解靶向嵌合体 (PROTACS)，以及线粒体自噬受体 Nix 与 LC3 和 GABARAP 的相互作用。它还有助于阐明蛋白质复合物中复杂的变构通讯，例如 trp RNA 结合衰减蛋白 (TRAP) 复合物。动力学分析的进步使得能够从预先存在的 ITC 数据集计算动力学速率常数。还开发了多种策略来研究酶动力学和酶抑制剂相互作用。ITC 还被应用于研究萃取、分离、和提纯应用，包括液-液分离和萃取蒸馏。从分析不同温度下的蛋白质不稳定性、测定活细胞悬浮液中的酶动力学到吸附水流中的尿毒症毒素，ITC 的各种应用已得到发展。

更新日期：2021-05-11

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