A synthetic acaricide, amitraz is widely used to control Varroa destructor. Although it has the potential to kill mites in honey bee colonies, the residue of amitraz and its metabolites in honey products is still of concern. Here, we determined the residue levels of amitraz and its metabolites in honey and beeswax when colonies were treated with different doses of Apivar^® (1X = 2 strips, 5X = 10 strips, and 10X = 20 strips); the 1X dose corresponds to a normal label-rate hive treatment. The results demonstrated that no amitraz residues were detected in honey and wax after 42 days of application in all treated colonies. The amitraz metabolites, 2,4-dimethylphenylformamide (DMPF) and 2,4-dimethyaniline (DMA), were found in the samples at 28 days post treatment. The residue levels of these two metabolites in beeswax were higher than those in honey. DMA was only detected in beeswax, ranging between 111 and 177 µg kg⁻¹, when colonies were treated with 5X and 10X of Apivar^®. The DMPF residue levels ranged between 13.7 and 60.5 µg kg⁻¹in honey and 196–6,160 µg kg⁻¹in wax samples. The residue of amitraz metabolites found in honey bee products did not exceed the maximum residue limits (MRLs), even though high doses were applied in this study to produce worse case scenarios.

一种合成的杀螨剂，双甲脒被广泛用于控制Varroa destructor。虽然它有可能杀死蜜蜂群落中的螨虫，但蜂蜜产品中的双甲脒及其代谢物的残留物仍然令人担忧。^{在这里，我们确定了用不同剂量的 Apivar ®}处理菌落时蜂蜜和蜂蜡中的双甲脒及其代谢物的残留水平（1X = 2 条，5X = 10 条，10X = 20 条）；1X 剂量对应于正常标记率的蜂巢治疗。结果表明，在所有处理过的菌落中施用 42 天后，在蜂蜜和蜡中未检测到双甲脒残留。在处理后 28 天的样品中发现了双甲脒代谢物 2,4-二甲基苯基甲酰胺 (DMPF) 和 2,4-二甲基苯胺 (DMA)。蜂蜡中这两种代谢物的残留水平高于蜂蜜中的残留水平。当用 5X 和 10X 的 Apivar ^®处理菌落时，仅在蜂蜡中检测到 DMA，范围在 111 和 177 µg kg ^-1之间。DMPF 残留水平在蜂蜜中介于 13.7 和 60.5 µg kg ^-1之间，在 196–6,160 µg kg ^-1之间在蜡样中。蜜蜂产品中发现的双甲脒代谢物残留未超过最大残留限量 (MRL)，尽管本研究中使用了高剂量以产生更糟糕的情况。