Propolis has been used by humans as a nutraceutical agent with therapeutic benefits for many centuries. The chemical composition and biological activities of propolis depend on its botanical and geographical origins. The aim of this study was to characterize Korean propolis by quantitating their chrysin content using an ultra high performance liquid chromatography (UPLC). Methanol extracts of propolis were comprehensively analyzed by UPLC for the isolation and quantification of chrysin in propolis from different origins. The reproducibility of the method was 1.9% to 6.0%, and accuracy was confirmed with average recovery ranging from 98.2% to 101.0%. The chrysin content in propolis samples was successfully determined within 13.299 min. All propolis samples, except those from Jeju Island, contained chrysin. There were some variations in the concentration of chrysin among propolis samples from different locations. The chrysin of the analyzed propolis samples confirmed their affiliation to the Korean propolis except those from Jeju Island, and the proposed UPLC method yielded good sensitivity, linearity, repeatability, and accuracy.

几个世纪以来，蜂胶一直被人类用作具有治疗功效的营养保健品。蜂胶的化学成分和生物活性取决于其植物和地理起源。本研究的目的是通过使用超高效液相色谱 (UPLC) 定量韩国蜂胶的白杨素含量来表征韩国蜂胶。采用UPLC对蜂胶甲醇提取物进行综合分析，对不同产地蜂胶中的白杨素进行分离和定量。该方法的重现性为1.9%至6.0%，准确度得到证实，平均回收率为98.2%至101.0%。13.299 分钟内成功测定蜂胶样品中的白杨素含量。除济州岛的蜂胶样品外，所有蜂胶样品都含有白杨素。不同产地的蜂胶样品中白杨素的浓度存在一定差异。分析的蜂胶样品中的白杨素证实了它们与除济州岛蜂胶之外的韩国蜂胶的关系，并且所提出的 UPLC 方法具有良好的灵敏度、线性、重复性和准确性。