ABSTRACT

In the current paper, NiO-NPs were synthesized through a sol–gel method by the usage of a biocompatible polymer known as Arabic gum (GA) as a stabilizing agent. The structural, optical, and magnetic properties of the obtained product was investigated by the application of TGA/DTA, FT-IR, UV-Vis, XRD, FESEM/EDX, and VSM analyzes. The FESEM images of NiO-NPs displayed its spherical shape and average size of about 59 nm. The antimicrobial activity of NiO-NPs was investigated against Gram-positive bacteria including Staphylococcus aureus, Bacillus subtilis and Gram-negative bacteria including Escherichia coli, Pseudomonas aeruginosa. The achieved findings indicated that all of the bacterial resistance was attributed to NiO-NPs, except for the case of Bacillus subtilis bacteria that did not grow at the concentration of 10 mM. We also evaluated the photocatalytic activity of nanoparticles in regards to the MB degradation and observed a photocatalytic degradation efficiency of about 80% throughout the results. The cytotoxicity of NiO-NPs has evaluated towards the cancer U87MG cell and normal Nervous Neurospheres (NCs) cells lines by means of MTT method. Detailed in vitro biological activities revealed the significant therapeutic potential of NiO-NPs. This product was able to exhibit potent cytotoxicity against U87MG cancer cells (IC₅₀: 37.84 μg mL⁻¹).

摘要

在当前的论文中，NiO-NPs是通过溶胶-凝胶法，通过使用一种生物相容性聚合物，即阿拉伯胶（GA）作为稳定剂来合成的。通过TGA / DTA，FT-IR，UV-Vis，XRD，FESEM / EDX和VSM分析研究了所得产物的结构，光学和磁性。NiO-NPs的FESEM图像显示其球形，平均尺寸约为59 nm。研究了NiO-NPs对革兰氏阳性菌（包括金黄色葡萄球菌，枯草芽孢杆菌）和革兰氏阴性菌（包括大肠杆菌，铜绿假单胞菌）的抗菌活性。取得的发现表明，除细菌外，所有细菌耐药性均归因于NiO-NPs。枯草芽孢杆菌细菌不能以10 mM的浓度生长。我们还评估了纳米粒子在MB降解方面的光催化活性，并在整个结果中观察到约80％的光催化降解效率。NiO-NPs的细胞毒性已经通过MTT方法评估了对癌细胞U87MG细胞和正常神经神经球（NCs）细胞的毒性。详细的体外生物学活性揭示了NiO-NP的重大治疗潜力。该产物能够表现出对U87MG癌细胞的有效细胞毒性（IC ₅₀：37.84μgmL ^-1）。