ABSTRACT

Ion substitution in hydroxyapatite (HA) lattice is a promising way to enhance its antibacterial activity and inhibit implant-related infections. In this study, HA/silver (HA/Ag) nanocomposites were synthesised through sol-gel method from two calcium (Ca) and phosphor (P) precursors. Our results showed that, by using P precursor, larger HA particles with higher crystallinity were produced, while Ca progenitor increased nucleation rate and created amorphous particles with smaller size. These findings were further confirmed by Fourier-transform infrared (FTIR) spectroscopy and X-ray diffraction (XRD) analysis. Moreover, starting with P precursors shifted the index plasmon peak of Ag to the higher wavelengths (500–550 nm), providing a higher tendency to release Ag⁺ ions and in vitro antibacterial activity at concentration of 800 μg/mL. As a result, this study proved that HA/Ag particles could be synthesised from P precursor with better phase structure and antibacterial activity, developed for biomedical applications especially as a biomimetic coating of bone implants.

摘要

羟基磷灰石 (HA) 晶格中的离子取代是增强其抗菌活性和抑制植入物相关感染的一种有前途的方法。在这项研究中，HA/银 (HA/Ag) 纳米复合材料通过溶胶-凝胶法由两种钙 (Ca) 和磷 (P) 前体合成。我们的结果表明，通过使用 P 前体，产生了具有更高结晶度的较大 HA 颗粒，而 Ca 前体提高了成核率并产生了尺寸更小的无定形颗粒。傅里叶变换红外 (FTIR) 光谱和 X 射线衍射 (XRD) 分析进一步证实了这些发现。此外，从 P 前体开始，将 Ag 的指数等离子体峰转移到更高的波长（500-550 nm），提供更高的释放 Ag ⁺离子的趋势和体外浓度为 800 μg/mL 时具有抗菌活性。因此，本研究证明 HA/Ag 颗粒可以由 P 前体合成，具有更好的相结构和抗菌活性，可用于生物医学应用，特别是作为骨植入物的仿生涂层。