Cell separation technique is essential for the detection of circulating tumor cells in blood and for enrichment of stem cell in regenerative medicine. However, conventional methods require cell labeling such as antibodies, which may damage the cells during the operation. Therefore, dielectrophoresis (DEP), a “label‐free” separation technique that eliminates the effects on cells, has been attracting attention. In this paper, we investigated the dielectric properties of Jurkat cells derived from human T‐cell leukemia cells by dielectrophoretic velocimetry. An equivalent circuit model of the cell was constructed and the frequency characteristics of the voltage of each layer of the cell were calculated. As a result, it was shown that the live and dead cells showed opposite dielectrophoretic forces in the region below 10 kHz, indicating the possibility of separating them. The agreement between the theoretical calculations and the experimental results shows the validity of the equivalent circuit model.

中文翻译：

---

介电测速法研究癌细胞介电性能的基础研究

细胞分离技术对于血液中循环肿瘤细胞的检测以及再生医学中干细胞的富集至关重要。但是，常规方法需要诸如抗体之类的细胞标记，这可能会在手术过程中损坏细胞。因此，介电泳（DEP）是一种消除了对细胞的影响的“无标记”分离技术，已经引起了人们的关注。在本文中，我们通过介电测速法研究了源自人T细胞白血病细胞的Jurkat细胞的介电特性。构建了电池的等效电路模型，并计算了电池每一层电压的频率特性。结果表明，在10 kHz以下的区域中，活细胞和死细胞显示出相反的介电泳力，表示有可能将它们分开。理论计算与实验结果之间的一致性证明了等效电路模型的有效性。