Acmella oleracea contains spilanthol as the main active compound, which possesses analgesic and anti-inflammatory effects that can favor tendon reorganization. To analyze the effect of A. oleracea on the content and organization of collagen in injured tendons, the calcaneal tendon of male Lewis rats was partially transected and treated at the site of injury with a topical application of 20% A. oleracea ointment (AO group) or with the ointment base without the plant extract (B group). The animals were euthanized 21 days after partial transection. Higher collagen concentration was observed in the AO group than in the B group, and morphological analysis using polarization microscopy showed higher birefringence in the AO group than in the B group, indicating higher collagen organization. No difference was observed in the number of fibroblasts, blood vessels, proteoglycan distribution, and maximum load between the B and AO groups. In conclusion, topical application of 20% A. oleracea ointment increased the molecular organization and content of collagen, thus indicating a potential application in tendon repair. Studies on the later phases of the tendon healing process are necessary to demonstrate the possible biomechanical changes after the application of A. oleracea ointment.

中文翻译：

---

Acmella oleracea 提取物增加部分横断肌腱的胶原蛋白含量和组织

Acmella oleracea含有千日菊醇作为主要活性化合物，具有镇痛和抗炎作用，可以促进肌腱重组。为了分析甘蓝对受伤肌腱中胶原蛋白含量和组织的影响，将雄性 Lewis 大鼠的跟腱部分切断并在损伤部位局部使用 20%甘蓝处理软膏（AO 组）或不含植物提取物的软膏基质（B 组）。部分横断后21天对动物实施安乐死。AO 组的胶原浓度高于 B 组，使用偏光显微镜的形态学分析显示 AO 组的双折射高于 B 组，表明胶原组织更高。B 组和 AO 组之间在成纤维细胞数量、血管、蛋白多糖分布和最大负荷方面没有观察到差异。总之，外用 20% A. oleracea软膏增加了胶原蛋白的分子组织和含量，从而表明在肌腱修复中的潜在应用。有必要对肌腱愈合过程的后期阶段进行研究，以证明使用A. oleracea软膏后可能发生的生物力学变化。