OsPho1 in Zhonghua 11 (ZH11) was edited using the clustered regularly interspaced short palindromic repeats- associated endonuclease 9 (CRISPR/Cas9) system. Two homozygous T₁ mutants (cr-pho1-34 and cr-pho1-37) displayed a chalky endosperm with a white core, which significantly decreased 1000-grain weight. In addition, many rounded starch granules and abnormal amyloplasts were present in the central region of mutant endosperm cells with increased amylose and lipid contents, decreased total protein content, and altered physicochemical properties of starch. The OsPho1 protein is localized in chloroplasts, and quantitative real-time PCR (qRT-PCR) and β-glucuronidase (GUS) staining indicated that OsPho1 was highly expressed in seeds at 5 d after fertilization (DAF). OsPho1 mutations displayed close relationships with plastidial phosphoglucomutase and ADP- Glc pyrophosphorylase based on α-D-glucose-1P at different temperatures. Moreover, the expressions of starch metabolism- related genes were also altered in the mutant, and the over- expression of OsPho1 may cause grain chalkiness.

OsPho1（ZH11）中花11是使用群集规则间隔开的短回文repeats-关联的核酸内切酶9（CRISPR / Cas9）系统进行编辑。两个纯合的T ₁突变体（cr-pho1-34和cr-pho1-37）显示出带有白色核心的白垩质胚乳，显着降低了1000粒重。此外，在突变的胚乳细胞的中央区域存在许多圆形的淀粉颗粒和异常的淀粉质体，其中直链淀粉和脂质含量增加，总蛋白含量降低，并且淀粉的理化特性发生了变化。OsPho1蛋白位于叶绿体中，并且实时定量PCR（qRT-PCR）和β-葡萄糖醛酸苷酶（GUS）染色表明OsPho1受精后第5天（DAF）的高表达在种子中表达。在不同温度下，OsPho1突变与基于α - D-葡萄糖-1P的质体磷酸葡萄糖突变酶和ADP-Glc焦磷酸化酶表现出密切的关系。此外，该突变体中淀粉代谢相关基因的表达也发生了改变，而OsPho1的过表达可能会导致谷物的垩白。