Lichens can produce secondary metabolites with important biological activities such as antioxidants, antibacterial, etc. Physodic acid (PA) is an important lichen secondary metabolite. The anticancer activity of PA has been shown in many tumor types other than lung cancer. The aim of this study was to investigate the effects of PA on A549 lung adenocarcinoma cells. In this study, A549 lung cancer cell line was used. MTT and colony formation assays were used to evaluate cytotoxic effects of PA at 24, 48, and 72 h; hematoxylin–eosin staining and DAPI staining were used for determining cell and nucleus morphology, respectively, and wound healing assay was used for investigating cell migration. PA was shown to be cytotoxic in 24, 48, and 72 h by increasing concentrations. IC50 concentrations at 24, 48, and 72 h were found to be 382.0, 235.4, and 175.8 μM, respectively. PA was found to inhibit colony formation completely at non-toxic concentration. It was observed that PA disrupted cell and nuclear morphology and showed apoptotic activities by increasing concentrations. It was determined that non-toxic and higher concentrations of PA inhibited cell migration widely. In conclusion, PA was shown to have cytotoxic, apoptotic and cell migration inhibitory effects on A549 lung adenocarcinoma cell line.

地衣可以产生具有重要生物活性的次生代谢产物，例如抗氧化剂，抗菌剂等。植酸（PA）是重要的地衣次生代谢产物。PA的抗癌活性已在除肺癌以外的许多其他类型的肿瘤中显示出来。这项研究的目的是研究PA对A549肺腺癌细胞的作用。在这项研究中，使用了A549肺癌细胞系。使用MTT和集落形成测定法评估PA在24、48和72小时的细胞毒性作用。苏木精-伊红染色和DAPI染色分别用于确定细胞和细胞核形态，伤口愈合测定用于研究细胞迁移。随着浓度的升高，PA在24、48和72小时内显示出细胞毒性。发现在24、48和72小时的IC50浓度为382.0、235.4和175。分别为8μM。发现PA在无毒浓度下完全抑制菌落形成。观察到PA通过增加浓度破坏细胞和核的形态，并显示出凋亡活性。已确定无毒和较高浓度的PA广泛抑制细胞迁移。总之，PA被证明对A549肺腺癌细胞系具有细胞毒性，凋亡和细胞迁移抑制作用。