Carbapenemase-producing organisms (CPOs) pose a serious clinical threat and rapid detection tools are essential to aid in patient management. We developed rapid and simple molecular tests to detect bla_NDM-type and bla_VIM-type carbapenemase genes using recombinase polymerase amplification (RPA) combined with a lateral flow detection. The tests could provide results in approximately 15 min when using DNA extracts, with limits of detection of 9.2 copies/μl for the bla_NDM-type assay and 7.5 copies/μl for bla_VIM-type assay, and successfully detected all isolates harbouring the carbapenemase encoding genes in a panel of 57 isolates. These RPA tests may be suitable for use in low-resource settings to tailor rapid implementation of infection control precautions and antibiotic stewardship.

产碳青霉烯酶的生物体 (CPO) 构成严重的临床威胁，快速检测工具对于帮助患者管理至关重要。我们开发了快速而简单的分子测试，使用重组酶聚合酶扩增 (RPA) 结合侧向流动检测来检测bla _{NDM 型}和bla _{VIM 型}碳青霉烯酶基因。使用 DNA 提取物时，测试可在大约 15 分钟内提供结果，bla _{NDM 型}检测的检测限为 9.2拷贝/μl，bla _{VIM 型}检测的检测限为7.5 拷贝/μl化，并成功检测到在 57 个分离株中含有碳青霉烯酶编码基因的所有分离株。这些 RPA 测试可能适用于资源匮乏的环境，以便快速实施感染控制预防措施和抗生素管理。