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Antifungal action of chitosan in combination with fungicides in vitro and chitosan conjugate with gallic acid on tomatoes against Botrytis cinerea
Biotechnology Letters ( IF 2.0 ) Pub Date : 2021-05-11 , DOI: 10.1007/s10529-021-03138-6
Natalia Karpova 1 , Balzhima Shagdarova 1 , Alexey Lunkov 1 , Alla Il'ina 1 , Valery Varlamov 1
Affiliation  

In the present work, a positive effect was obtained by using low molecular weight chitosan compounds in combination with synthetic fungicides. Antifungal activity against Botrytis cinerea, determined by the radial growth method, was more than 75%, with a 25 × 10− 10 g/L concentration of fludioxonil or difenoconazole in compounds. Metabolic activity of B. cinerea fungus was about 15% when using a chitosan compound containing fludioxonil at a concentration of 25 × 10− 7 g/L. The combined action of chitosan with difenoconazole at a fungicide concentration of 25 × 10− 4 g/L is 2–3 times more effective than the action of each component separately. Results of studies for artificially inoculated B. cinerea tomato fruit when treated with low molecular chitosan and chitosan conjugate with gallic acid reduced the frequency of rotting fruit by 50 and 83%, respectively. Chitosan-gallic acid conjugate were obtained from chitosans with Mw of 28 kDa (Ch28GA) was proved to be effective as a preventive treatment for 3 days and can potentially be used as a biofungicide against B. cinerea on tomatoes in the post-harvest period.



中文翻译:

壳聚糖与杀菌剂组合的体外抗真菌作用以及壳聚糖与没食子酸偶联物对番茄对灰葡萄孢菌的抗真菌作用

在目前的工作中,通过将低分子量壳聚糖化合物与合成杀菌剂结合使用,获得了积极的效果。对灰霉病菌的抗真菌活性,通过径向生长法测定,超过 75%,化合物中氟菌腈或苯醚菌灵的浓度为25 × 10 - 10 g/L。当使用含有浓度为 25 × 10 − 7 g/L 的氟啶虫胺的壳聚糖化合物时,B. cinerea真菌的代谢活性约为 15% 。杀菌剂浓度为 25 × 10 - 4 g/L的壳聚糖与苯醚甲环唑的联合作用比每种成分单独作用的效果高 2-3 倍。人工接种的研究结果B. cinerea番茄果实在用低分子壳聚糖和壳聚糖与没食子酸共轭物处理后,果实腐烂频率分别降低了 50% 和 83%。从 Mw 为 28 kDa 的壳聚糖 (Ch28GA) 中获得的壳聚糖 - 没食子酸结合物被证明可有效作为 3 天的预防性处理,并且有可能在收获后用作抗B. cinerea的生物杀菌剂。

更新日期:2021-05-11
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