Sensitive and rapid tests of Escherichia coli drug sensitivity is very important for health of human and animals. An E. coli immunosensor was built based on electrochemical detection and immune detection technologies, through pretreating screen-printed electrodes, and analyzing the optimal reaction concentration of antigen antibody binding with the AC impedance method. Based on the detection system combining the immunosensor and electrochemical workstation, tests were carried out to measure the accuracy of E. coli concentration and drug sensitivity, and error of the detection system was calibrated in accordance with data from the electrochemical workstation. E. coli O157:H7 can be detected in the range of 10³ cfu/ml ~ 10¹² cfu/ml, and the detection error controlled within 5%. Results from the electrochemical workstation and those from the detection device were consistent, and both demonstrated a greater inhibitory effect of antibiotics on E. coli than on Bacillus subtilis. The electrochemical detection system is highly efficient and accurate, and could be widely applied to E. coli drug sensitivity tests in clinical medicine.

灵敏且快速的大肠杆菌药物敏感性测试对于人类和动物的健康非常重要。的大肠杆菌免疫是基于电化学检测和免疫检测技术，通过预处理丝网印刷电极，和分析抗原抗体与AC阻抗方法结合的最佳反应浓度建。以免疫传感器与电化学工作站相结合的检测系统为基础，对大肠杆菌浓度的准确性和药物灵敏度进行了测试，并根据电化学工作站的数据对检测系统的误差进行了校准。E.coli O157:H7 可检测范围为 10 ³ cfu/ml ~ 10¹² cfu/ml，检测误差控制在5%以内。电化学工作站的结果与检测装置的结果一致，均表明抗生素对大肠杆菌的抑制作用大于对枯草芽孢杆菌的抑制作用。该电化学检测系统高效、准确，可广泛应用于临床医学中大肠杆菌药敏试验。