Two new species of Viannaia from the intestine of the North American opossums, Didelphis virginiana (Virginia opossum), and Philander opossum (gray four-eyed opossum), are described based on morphological and molecular data, through an integrative taxonomic approach. Maximum likelihood and Bayesian inference analyses for each dataset and the concatenated dataset were performed using a mitochondrial cytochrome c oxidase subunit 1 (COI) gene, and the nuclear ribosomal internal transcribed spacer region (ITS1–5.8S–ITS2). The phylogenetic analyses revealed 2 new species that occur in Mexico, one from the western state of Colima and another from the southern state of Chiapas. Our phylogenetic trees for both molecular markers and concatenated datasets yielded similar topologies with high bootstrap values and posterior probabilities. Viannaia is recovered as a monophyletic group, but the family Viannaiidae appears as non-monophyletic, due to the position of Travassostrongylus scheibelorum, similar to previous studies. Finally, the morphology of Viannaia and Hoineffia is discussed.

基于形态学和分子数据，通过综合分类学方法描述了来自北美负鼠肠道的两个新品种Viannaia，Didelphis virginiana（弗吉尼亚负鼠）和Philander 负鼠（灰色四眼负鼠）。使用线粒体细胞色素c氧化酶亚基 1 ( COI ) 基因和核糖体内部转录间隔区 ( ITS1 – 5.8S – ITS2 )对每个数据集和串联数据集进行最大似然和贝叶斯推理分析）。系统发育分析揭示了墨西哥出现的 2 个新物种，一个来自西部科利马州，另一个来自南部恰帕斯州。我们的分子标记和连接数据集的系统发育树产生了具有高自举值和后验概率的类似拓扑。Viannaia被恢复为单系群，但 Viannaiidae 科由于Travassostrongylus scheibelorum的位置而表现为非单系，与之前的研究相似。最后，讨论了Viannaia和Hoineffia的形态。