Enzyme immobilization is necessary process to improve the bioactivity and stability of the biocatalyst. In this study, glucose oxidase (GOx) enzyme was immobilized on plasma-treated fibrous carbon felt as a textile carrier to produce a heterogeneous catalyst. Genipin, as a naturally occurring crosslinker, that has less cytotoxicity than conventional crosslinkers, was used in the enzyme immobilization process. UV-Vis and FTIR spectra confirmed the crosslinking reaction between genipin and the primary amines of GOx enzyme, by forming blue-pigmented aggregates. GOx relative activity after crosslinking and immobilization on the carbon felt was maintained up to 40%, with stability in performance up to 6 cycles for the plasma treated carbon, while maintaining their bio-electro-activity as shown from cyclic voltammetry scans (CV). The obtained heterogeneous catalysts have been tested for use in sustainable wastewater treatment of Remazol Blue RR (RB) dyestuff by means of Bio-Fenton (BF) and enzymatic Bio-Electro-Fenton (BEF) processes. The produced samples resulted in high color removal efficiency, up to 93% discoloration of (RB) for the first use in (BF) process in 3 h. Meanwhile, enzymatic (BEF) process resulted in up to 34% of COD removal, with simultaneous power density generation up to 0.16 ± 0.01 μW.cm^-2 at a current density of around 10 ± 2 μA.cm^-2 in 12 h. These results highlight the importance of genipin as a bio-based crosslinker for enzymes, and the potential use in both (BF) and (BEF) as sustainable approaches for wastewater treatment and as a step towards zero-energy degradation of organic matter.

固定酶是改善生物催化剂的生物活性和稳定性的必要过程。在这项研究中，将葡萄糖氧化酶（GOx）酶固定在经过等离子体处理的纤维碳毡上，将其作为纺织品载体生产非均相催化剂。Genipin是一种天然存在的交联剂，比常规交联剂具有更低的细胞毒性，被用于酶的固定过程。UV-Vis和FTIR光谱通过形成蓝色颜料聚集体，证实了Genipin和GOx酶的伯胺之间的交联反应。交联和固定在碳毡上后，GOx相对活性可保持高达40％，等离子体处理的碳的性能可保持高达6个循环的稳定性，同时保持其生物电活性，如循环伏安扫描（CV）所示。已通过生物芬顿（BF）和酶促生物电子芬顿（BEF）工艺对获得的多相催化剂进行了测试，以用于Remazol Blue RR（RB）染料的可持续废水处理。所生产的样品具有很高的脱色效率，在3中首次用于（BF）工艺中时（RB）的变色率高达93％ H。同时，酶（BEF）过程导致高达COD去除率为34％，并同时功率密度可以产生最高至0.16±0.01μW.cm ^-2在大约10±2μA.cm的电流密度^-2在12 小时。这些结果凸显了Genipin作为酶的生物基交联剂的重要性，以及在（BF）和（BEF）中的潜在用途作为废水处理的可持续方法以及迈向零能量降解有机物的一步。