Background Glioma is the most frequent, highly aggressive primary intracranial malignant tumor. Circular RNA (circRNA) circ_0037655 has been reported to be a vital regulator in glioma. The different functional mechanism behind circ_0037655 was investigated in the current study. Methods The expression of circ_0037655, microRNA-1229-3p (miR-1229-3p) and integrin beta-8 (ITGB8) was detected via the quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Cellular research was performed via colony formation assay for cell proliferation, flow cytometry for cell cycle and cell apoptosis, scratch assay for cell migration, as well as transwell assay for cell migration and invasion. Western blot was used for detection of ITGB8 protein and epithelial–mesenchymal transition (EMT) process. Dual-luciferase reporter assay was implemented for the binding analysis of potential targets. In vivo assay was administered via xenograft in mice. Results Upregulation of circ_0037655 was affirmed in glioma samples and cells. Tumor formation and metastasis of glioma were inhibited after circ_0037655 was downregulated. miR-1229-3p acted as a target of circ_0037655, and its upregulation was responsible for the function of si-circ_0037655 in glioma cells. miR-1229-3p functioned as a tumor inhibitor in glioma progression by targeting ITGB8. circ_0037655 modulated the ITGB8 expression by targeting miR-1229-3p. In vivo knockdown of circ_0037655 also suppressed glioma tumorigenesis by acting on the miR-1229-3p/ITGB8 axis. Conclusion This study showed that downregulation of the expression of circ_0037655 could inhibit glioma progression by acting on the miR-1229-3p/ITGB8 axis. The specific circ_0037655/miR-1229-3p/ITGB8 axis was disclosed in glioma research.

中文翻译：

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circ_0037655的下调通过miR-1229-3p / ITGB8轴的调节阻止神经胶质瘤的形成和转移

背景胶质瘤是最常见，高度侵袭性的原发性颅内恶性肿瘤。据报道，环状RNA（circRNA）circ_0037655是神经胶质瘤的重要调节剂。在当前研究中，研究了circ_0037655背后的不同功能机制。方法通过定量逆转录-聚合酶链反应（qRT-PCR）检测circ_0037655，microRNA-1229-3p（miR-1229-3p）和整联蛋白β-8（ITGB8）的表达。细胞研究通过集落形成分析进行细胞增殖，流式细胞术进行细胞周期和细胞凋亡，划痕分析进行细胞迁移，以及transwell分析进行细胞迁移和侵袭。免疫印迹用于检测ITGB8蛋白和上皮-间质转化（EMT）过程。实施了双重荧光素酶报告基因分析，用于潜在靶标的结合分析。通过异种移植在小鼠中进行体内测定。结果在神经胶质瘤样品和细胞中证实了circ_0037655的上调。circ_0037655下调后，神经胶质瘤的形成和转移受到抑制。miR-1229-3p充当circ_0037655的靶标，其上调负责si-circ_0037655在神经胶质瘤细胞中的功能。miR-1229-3p通过靶向ITGB8，在神经胶质瘤进展中起肿瘤抑制剂的作用。circ_0037655通过靶向miR-1229-3p来调节ITGB8表达。circ_0037655的体内敲除还通过作用于miR-1229-3p / ITGB8轴来抑制神经胶质瘤的发生。结论这项研究表明，下调circ_0037655的表达可以通过作用于miR-1229-3p / ITGB8轴来抑制神经胶质瘤的进展。在神经胶质瘤研究中公开了特定的circ_0037655 / miR-1229-3p / ITGB8轴。