This study presents an effective high-speed countercurrent chromatography method of two-phase solvent system combined with ionic liquids for the isolation and purification of four major compounds of γ-oryzanol from rice bran oil. The conditions of high-speed countercurrent chromatography, including solvent systems and the content of ionic liquids, were optimized to improve separation efficiency. The optimal solvent system was n-hexane–dichloromethane–acetonitrile–[C₄mim][PF₆] at a volume ratio of (10:1:10:0.15, v/v). Adding a small amount of ionic liquids significantly improved the separation efficiency. Four compounds of γ-oryzanol, cycloartenyl ferulate, 24-methylene cycloartanyl ferulate, campesteryl ferulate, and sitosteryl ferulate were successfully purified in one run from rice bran oil with the purities of 95.25%, 92.48%, 92.47%, and 93.43%, respectively. The result of bioassay showed that rice bran oil and separated individual cycloartenyl ferulate could significantly inhibit mycelium growth of crop diseases Rhizoctonia solani and Fusarium graminerum, the bioactivity of cycloartenyl was higher than that of the rice bran oil. The cellular antioxidant activity experimental result indicted that cycloartenyl ferulate could alleviate H₂O₂-induced oxidative stress.

本研究提出了一种有效的高速两相溶剂系统与离子液体相结合的逆流色谱方法，用于从米糠油中分离和纯化γ-谷维素的四种主要化合物。优化了高速逆流色谱的条件，包括溶剂体系和离子液体的含量，以提高分离效率。最佳溶剂体系为正己烷-二氯甲烷-乙腈-[C ₄ mim] [PF ₆体积比为（10：1：10：0.15，v / v）。添加少量的离子液体显着提高了分离效率。从一次米糠油中一次纯化成功地纯化了四种化合物-γ-谷维素，阿魏酸环戊烯基，阿魏酸24-亚甲基环戊基酯，阿魏酸菜油酸酯和谷甾醇阿魏酸酯，其纯度分别为95.25％，92.48％，92.47％和93.43％。 。生物测定结果表明，米糠油和分离的单个阿魏酸环烯基酯能显着抑制农作物病原菌丝状镰刀菌和禾谷镰刀菌的菌丝生长，环戊烯基的生物活性高于米糠油。细胞抗氧化活性实验结果表明阿魏酸环戊烯酯可以缓解H ₂O ₂引起的氧化应激。