Horizontal transfer of domains in ssrA gene among Enterobacteriaceae,Genes to Cells

当前位置： X-MOL 学术 › Genes Cells › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Horizontal transfer of domains in ssrA gene among Enterobacteriaceae
Genes to Cells ( IF 1.3 ) Pub Date : 2021-05-10 , DOI: 10.1111/gtc.12869
Nagarajan Tamilmaran ₁ , Ramamoorthy Sankaranarayanan ₂ , Punitha Selvakumar A S ₃ , M Hussain Munavar ₁

Affiliation

The tmRNA (transfer messenger RNA), encoded by ssrA gene, is involved in rescuing of stalled ribosomes by a process called trans-translation. Additionally, regions of the ssrA gene (coding for tmRNA) were reported to serve as integration sites for various bacteriophages. Though variations in ssrA genes were reported, their functional relevance is less studied. In this study, we investigated the horizontal gene transfer (HGT) of ssrA among the members of Enterobacteriaceae. This was done by predicting recombination signals in ssrA gene (belonging to Enterobacteriaceae) using RDP5 (Recombination Detection Program 5). Our results revealed 7 recombination signals in ssrA gene belonging to different species. We further showed that the recombination signals were more in the domains present in the 3′ end than 5′ end of tmRNA. Of note, the mRNA region was reported in many recombination signals. Further, members belonging to genera Yersinia, Erwinia, Dickeya and Enterobacter were highly represented in the recombination signals. Sequence analysis revealed the presence of integration sites for different class of bacteriophages in ssrA gene. The locations of phage recognition sites are comparable with recombination signals. Taken together, our results revealed a diverse nature of HGT and recombination which possibly due to transduction mediated by phages.

中文翻译：

肠杆菌科 ssrA 基因域的水平转移

由ssrA基因编码的 tmRNA（转移信使 RNA）参与通过称为反式翻译的过程拯救停滞的核糖体。此外，据报道ssrA基因（编码 tmRNA）的区域可作为各种噬菌体的整合位点。尽管报告了ssrA基因的变异，但它们的功能相关性研究较少。在这项研究中，我们调查了肠杆菌科成员中ssrA的水平基因转移 (HGT) 。这是通过使用 RDP5（重组检测程序 5）预测ssrA基因（属于肠杆菌科）中的重组信号来完成的。我们的结果揭示了ssrA 中的7 个重组信号属于不同物种的基因。我们进一步表明，重组信号更多地出现在 tmRNA 的 3' 端而不是 5' 端。值得注意的是，mRNA 区域在许多重组信号中都有报道。此外，属于耶尔森氏菌属、欧文氏菌属、Dickeya属和肠杆菌属的成员在重组信号中具有很高的代表性。序列分析揭示了ssrA基因中存在不同种类噬菌体的整合位点。噬菌体识别位点的位置与重组信号相当。总之，我们的结果揭示了 HGT 和重组的多样性，这可能是由于噬菌体介导的转导所致。

更新日期：2021-07-09

点击分享查看原文

点击收藏

公开下载

阅读更多本刊最新论文本刊介绍/投稿指南11