Background

Acute coronary syndrome (ACS) is a complex cardiovascular disease whose development involves the dysregulation of adaptive immune responses. Though it has been proven that T cells associate with inflammation in the development of ACS, the function of B cells in disease remains unclear.

Objective

The aim of this study was to reveal the diversity of the B cell receptor (BCR) repertoire of patients with ACS.

Methods

We conducted a pilot study to sequence the immune repertoire of peripheral blood mononuclear cells (PBMCs) from patients with ACS, including acute myocardial infarction (AMI) and unstable angina (UA), and quantitatively characterized BCR repertoires by bioinformatics analysis.

Results

We found that patients with AMI and UA had lower BCR repertoire diversity compared with controls with normal coronary arteries (NCA). Lower percentages of productive unique BCR nt sequences and higher percentages of top 200 unique BCR sequences were identified in AMI and UA patients than NCA controls. Patients had various preferential usage of V and J genes from B cell clones in accordance with the disease severity of coronary arteries. AMI patients had distinct CDR3 amino acids, and their frequency differed among patients with ACS.

Conclusions

Our results indicate that differential BCR signatures represent an imprint of distinct repertoires among ACS patients. This study thereby opens up the prospect of studying disease-relevant B cells to better understand and treat ACS.

中文翻译：

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急性冠状动脉综合征患者 B 细胞受体库的特征

背景

急性冠状动脉综合征（ACS）是一种复杂的心血管疾病，其发展涉及适应性免疫反应的失调。尽管已证明 T 细胞与 ACS 发展过程中的炎症有关，但 B 细胞在疾病中的功能仍不清楚。

客观的

本研究的目的是揭示 ACS 患者 B 细胞受体 (BCR) 库的多样性。

方法

我们进行了一项初步研究，对 ACS 患者外周血单个核细胞 (PBMC) 的免疫库进行测序，包括急性心肌梗死 (AMI) 和不稳定型心绞痛 (UA)，并通过生物信息学分析定量表征 BCR 库。

结果

我们发现，与冠状动脉正常 (NCA) 的对照组相比，AMI 和 UA 患者的 BCR 曲目多样性较低。与 NCA 对照相比，在 AMI 和 UA 患者中鉴定出的生产性独特 BCR nt 序列百分比较低，而前 200 个独特 BCR 序列百分比较高。根据冠状动脉疾病的严重程度，患者对来自 B 细胞克隆的 V 和 J 基因有多种优先使用。AMI 患者具有不同的 CDR3 氨基酸，其频率在 ACS 患者中有所不同。

结论

我们的结果表明，不同的 BCR 特征代表了 ACS 患者中不同曲目的印记。因此，这项研究开辟了研究疾病相关 B 细胞以更好地了解和治疗 ACS 的前景。