Aloperine has been shown to exhibit tremendous pharmacological potential. The present study was designed to investigate the effects of aloperine against human liver cancer cells and to explore the underlying mechanism. The results showed significant (P < .05) upregulation of GROα (GRO1 oncogene) in liver cancer tissues and cell lines. However, the expression of GROα significantly (P < .05) declined in liver cancer cells treated with aloperine (5 µM) which was concomitant with the inhibition of proliferation. Silencing of GROα significantly (P < .05) inhibited the proliferation of the liver cancer via G₂/M cycle arrest. Interestingly, aloperine also triggered G₂/M cell cycle arrest of the liver cancer cells. Nonetheless, the growth inhibitory effects of aloperine on liver cancer cells were attenuated by GROα overexpression and the cancer cells did not show the arrest of cell division. Additionally, aloperine also suppressed the migration, invasion and epithelial to mesenchymal transition of the human liver cancer cells via inhibition of GROα expression. Collectively, the findings revealed growth inhibitory effects of aloperine via suppression of GROα expression and point toward its therapeutic potential.

已证明阿罗匹林具有巨大的药理潜力。本研究旨在研究苦参碱对人肝癌细胞的作用并探讨其潜在机制。结果表明 ，肝癌组织和细胞系中的GROα（GRO1癌基因）显着上调（P <.05）。然而，在 用苦参碱（5 µM）处理的肝癌细胞中，GROα的表达显着下降（P <.05），这与增殖的抑制相伴。 通过G ₂ / M周期阻滞，GROα的沉默显着（P <.05）抑制了肝癌的增殖。有趣的是，苦参碱还触发了G ₂/ M细胞周期阻滞肝癌细胞。然而，GROα过表达减弱了苦豆碱对肝癌细胞的生长抑制作用，并且癌细胞没有显示出细胞分裂的停止。另外，苦参碱还通过抑制GROα表达来抑制人肝癌细胞的迁移，侵袭和上皮向间质转化。总的来说，该发现揭示了通过抑制GROα表达而抑制了苦参碱的生长，并指出了其治疗潜力。