This study aimed to investigate the effect of the miR-29c/PTEN axis on the PI3 K/Akt/NF-kB pathway in rats with severe pneumonia (SP). A total of 20 Sprague–Dawley rats were selected, of which 10 were randomly selected for modeling SP and included in the model group (MG) and the other 10 were included in the control group (CG). Expression levels of miR-29c and PTEN were measured in the peripheral blood and lung tissue. Pearson correlation coefficient analysis showed that miR-29c was positively correlated with PTEN in serum and lung tissue (r = 0.833 and 0.697, respectively; P < 0.05). The apoptosis rate was higher in the MG than in the CG (P < 0.05). Western blot analysis revealed that the relative expressions of PI3 K, Akt, and NF-kB proteins were higher in the MG than in the CG (P < 0.05). Protein expression in the miR-29c-mimics and sh-PTEN groups was higher than that in the other two groups, whereas the miR-29c-inhibitor group had the lowest protein expression (P < 0.05). MiR-29c/PTEN exhibited low expression levels in SP, which can promote pneumonia development by inhibiting the expression of PI3 K/Akt/NF-kB pathway proteins.

这项研究旨在研究miR-29c / PTEN轴对重症肺炎（SP）大鼠PI3 K / Akt / NF-kB途径的影响。总共选择了20只Sprague-Dawley大鼠，其中10只随机选择用于模型SP，并包括在模型组（MG）中，另10只包括在对照组（CG）中。在外周血和肺组织中测量了miR-29c和PTEN的表达水平。Pearson相关系数分析显示，miR-29c与血清和肺组织中的PTEN正相关（r分别为0.833和0.697；P  <0.05）。MG的细胞凋亡率高于CG的细胞（P  <0.05）。蛋白质印迹分析显示，MG中PI3 K，Akt和NF-kB蛋白的相对表达高于CG（P  <0.05）。miR-29c-mimics和sh-PTEN组的蛋白表达高于其他两组，而miR-29c-抑制剂组的蛋白表达最低（P  <0.05）。MiR-29c / PTEN在SP中表现出低表达水平，可通过抑制PI3 K / Akt / NF-kB途径蛋白的表达来促进肺炎的发展。