This study aimed to explore the effect of the immunopotentiator CVC1302 on foot-and-mouth disease (FMD) vaccination in animals placed under oxidative stress. We established oxidative stress models using porcine circovirus type 2 (PCV2)-infected PK-15 cells and mice model both in vitro and in vivo, respectively. The efficacy of CVC1302 on PK-15 cells or in addition to the FMD vaccine was evaluated by quantitative real-time polymerase chain reaction, histopathological and enzyme-linked immunosorbent assay (ELISA) analysis. CVC1302 affected apoptosis of PCV2-infected PK-15 cells and significantly inhibited PCV2 replication, while it had no effect on the viability for blank PK-15 cell in vitro test with varying dilutions of CVC1302. Results showed that PCV2 induced a strong oxidative stress response in mice. CVC1302 reduced the viral load in spleen of PCV2-infected mice and ameliorated the pathological injury of spleen. Furthermore, CVC1302 significantly increased IgG antibody titer, cytokine expression, superoxide dismutase activity, catalase concentrations, and glutathione content in mice immunized with FMD vaccine. In conclusion, CVC1302 inhibits PCV2 replication and regulates oxidative stress in PCV2-infected mice, which can improve the immune efficacy of the FMD vaccine, providing a safe and effective immune enhancement.

中文翻译：

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评估 CVC1302 提高口蹄疫灭活疫苗在 PCV2 感染产生的氧化应激小鼠中的功效

本研究旨在探讨免疫增强剂 CVC1302 对置于氧化应激下的动物口蹄疫 (FMD) 疫苗接种的影响。我们分别使用猪圆环病毒 2 型 (PCV2) 感染的 PK-15 细胞和小鼠模型在体外和体内建立了氧化应激模型。通过定量实时聚合酶链反应、组织病理学和酶联免疫吸附试验 (ELISA) 分析评估 CVC1302 对 PK-15 细胞或除 FMD 疫苗之外的功效。CVC1302影响PCV2感染的PK-15细胞凋亡并显着抑制PCV2复制，而对体外空白PK-15细胞活力无影响使用不同稀释度的 CVC1302 进行测试。结果表明，PCV2 在小鼠体内诱导了强烈的氧化应激反应。CVC1302降低了PCV2感染小鼠脾脏中的病毒载量，改善了脾脏的病理损伤。此外，CVC1302 显着增加了口蹄疫疫苗免疫小鼠的 IgG 抗体滴度、细胞因子表达、超氧化物歧化酶活性、过氧化氢酶浓度和谷胱甘肽含量。总之，CVC1302抑制PCV2复制并调节PCV2感染小鼠的氧化应激，可提高口蹄疫疫苗的免疫功效，提供安全有效的免疫增强。