Abstract

Modern endometritis therapeutics may require an extremely precise and controlled drug release system. Poly lactic-co-glycolic acid (PLGA) microspheres loaded with two different drugs like amoxicillin and vancomycin were prepared and their efficacy in management of endometritis in murine model was examined. Such biological nanocarriers are need of the hour since they are biodegradable, cytocompatible, hemocompatible and easily formulated. The sizes of the drug loaded PLGA microspheres ranged from 65–100 nm. The electron microscopy images depicted them as uniform spherical structures. The zeta potential and polydispersity index were calculated and the in vitro release investigation indicated steady drug release over a period of 50 h at effective dosage. The drug loaded nanocarriers were found with be cytocompatible and hemolysis test elaborated that they met with hemolysis rate safety requirements. The murine models were induced with endometritis by intravaginal administration of bovine uterine isolates. The mice were sacrificed and histopathogical examination of the endometrial slides stained with haematoxylin and eosin revealed that the drug loaded PLGA nanospheres helped in reducing acute endometritis in mice in a very short time. This study thereby demonstrated well described synthesis approach of drug loaded PLGA nanospheres which may be further be transferred to preclinical laboratory studies.

摘要

现代子宫内膜炎治疗剂可能需要极其精确和受控的药物释放系统。制备了载有两种不同药物（如阿莫西林和万古霉素）的聚乳酸-乙醇酸（PLGA）微球，并检查了它们在小鼠模型中治疗子宫内膜炎的功效。此类生物纳米载体是小时所需的，因为它们是可生物降解的，细胞相容的，血液相容的并且易于配制。载有药物的PLGA微球的尺寸范围为65-100 nm。电子显微镜图像将它们描绘为均匀的球形结构。计算ζ电势和多分散指数，并在体外释放研究表明，在有效剂量下，药物可在50小时内稳定释放。发现载有药物的纳米载体具有细胞相容性，并进行了溶血试验，证明它们符合溶血率安全性要求。通过子宫内分离牛子宫分离物子宫内膜炎诱导鼠模型。处死小鼠并用苏木精和曙红染色的子宫内膜玻片的组织病理学检查显示，载有药物的PLGA纳米球有助于在很短的时间内减少小鼠的急性子宫内膜炎。因此，该研究证明了载药量很高的PLGA纳米球的合成方法，可以进一步转移到临床前实验室研究中。