3D models are increasingly used to study mechanisms driving tumor progression and mimicking in vitro processes such as invasion and migration. However, there is a need to establish more protocols based on 3D culture systems that allow for downstream molecular biology investigations. Materials & methods: Here we present a method for optimal RNA extraction from highly aggressive primary glioma cells invading into Matrigel. The method has been established by comparing previously reported protocols, available commercial kits and optimizing specific steps for matrix dissociation, RNA separation and purification. Results and conclusion: The protocol allows RNA extraction from cells embedded into Matrigel, with optimal yield, purity and integrity suitable for subsequent sequencing analysis of both high and low molecular weight RNA.

中文翻译：

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肿瘤细胞侵入基质胶：优化的 RNA 提取方案。

3D 模型越来越多地用于研究驱动肿瘤进展和模拟体外过程（如侵袭和迁移）的机制。然而，需要建立更多基于 3D 培养系统的协议，以进行下游分子生物学研究。材料和方法：在这里，我们提出了一种从侵入基质胶的高侵袭性原代神经胶质瘤细胞中提取最佳 RNA 的方法。该方法是通过比较先前报道的协议、可用的商业试剂盒和优化基质解离、RNA 分离和纯化的特定步骤而建立的。结果与结论： 该协议允许从嵌入 Matrigel 的细胞中提取 RNA，具有适合后续高分子量和低分子量 RNA 测序分析的最佳产量、纯度和完整性。