SummaryRegulation of the transforming growth factor beta (TGFβ) superfamily by gonadotrophins in swine follicular cells is not fully understood. This study evaluated the expression of steroidogenic enzymes and members of the TGFβ superfamily in prepubertal gilts allocated to three treatments: 1200 IU eCG at D −3 (eCG); 1200 IU eCG at D −6 plus 500 IU hCG at D −3 (eCG + hCG); and the control, composed of untreated gilts. Blood samples and ovaries were collected at slaughter (D0) and follicular cells were recovered thereafter. Relative gene expression was determined by real-time PCR. Serum progesterone levels were greater in the eCG + hCG group compared with the other groups (P < 0.01). No differences were observed in the expression of BMP15, BMPR1A, BMPR2, FSHR, GDF9, LHCGR and TGFBR1 (P > 0.05). Gilts from the eCG group presented numerically greater mean expression of CYP11A1 mRNA than in the control group that approached statistical significance (P = 0.08) and greater expression of CYP19A1 than in both the eCG and the control groups (P < 0.05). Expression of BMPR1B was lower in the eCG + hCG treatment group compared with the control (P < 0.05). In conclusion, eCG treatment increased the relative expression of steroidogenic enzymes, whereas treatment with eCG + hCG increased serum progesterone levels. Although most of the evaluated TGFβ members were not regulated after gonadotrophin treatment, the downregulation of BMPR1B observed after treatment with eCG + hCG and suggests a role in luteinization regulation.

中文翻译：

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具有不同内分泌特征的青春期前母猪滤泡细胞中类固醇生成酶和TGFβ超家族成员的表达

总结 促性腺激素对猪滤泡细胞中转化生长因子 β (TGFβ) 超家族的调节作用尚不完全清楚。该研究评估了分配给三种处理的青春期前母猪中类固醇生成酶和 TGFβ 超家族成员的表达：1200 IU eCG at D -3 (eCG)；D -6 时 1200 IU eCG 加上 D -3 时 500 IU hCG（eCG + hCG）；和对照组，由未经处理的后备母猪组成。在屠宰场（D0）收集血样和卵巢，然后回收卵泡细胞。通过实时PCR确定相对基因表达。与其他组相比，eCG + hCG 组的血清孕酮水平更高（磷< 0.01)。的表达没有观察到差异BMP15,BMPR1A,BMPR2,FSHR,GDF9,长对撞机和TGFBR1(磷> 0.05)。来自心电图组的后备母猪在数值上表现出更高的平均表达CYP11A1mRNA比对照组接近统计学意义（磷= 0.08）和更大的表达CYP19A1比心电图组和对照组（磷< 0.05)。的表达BMPR1B与对照组相比，eCG + hCG 治疗组较低（磷< 0.05)。总之，eCG 治疗增加了类固醇生成酶的相对表达，而用 eCG + hCG 治疗增加了血清孕酮水平。尽管大多数评估的 TGFβ 成员在促性腺激素治疗后没有受到调节，但BMPR1B在用 eCG + hCG 治疗后观察到，表明在黄体化调节中起作用。