The current study investigated the effects of the inclusion of modern hybrid rye (Brasetto variety) to a corn–wheat-based diet, with or without xylanase, on the absorptive surface of the small intestine of broilers. A total of 224 one-day-old male Ross 308 broiler chicks were randomly divided into four experimental groups with seven replicate cages of eight birds/replicate. A 2 × 2 factorial study design was used, with rye inclusion (0% or 20%) and xylanase supplementation (0 or 200 mg/kg of feed) as factors. Inclusion of rye increased duodenal and ileal crypt depth, villi height, the villus-to-crypt ratio and absorption surface area (p < 0.05), and ileal mucosa thickness and crypt width (p < 0.05). Xylanase supplementation attenuated the effects of rye in the duodenum and ileum and decreased the villi height and villus-to-crypt ratio in the jejunum (p < 0.05). Rye and xylanase had no effect on the spatial distribution of claudin 3 and ZO-1 protein, but xylanase supplementation reduced the amount of claudin 3 in the duodenum and jejunum (p < 0.05). The findings of this study indicate that 20% inclusion of modern hybrid rye to the diets of broilers improved the structure of the duodenum and ileum, but these effects were attenuated by xylanase supplementation.

中文翻译：

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现代杂交黑麦作为肉鸡的替代能源，依靠肠道部分和补充木聚糖酶来改善小肠的吸收表面


目前的研究调查了在玉米-小麦为主的日粮中添加现代杂交黑麦（Brasetto 品种）（含或不含木聚糖酶）对肉鸡小肠吸收表面的影响。总共 224 只一日龄雄性 Ross 308 肉鸡被随机分为 4 个实验组，每个实验组有 7 个重复笼，每组 8 只鸡。采用 2 × 2 析因研究设计，以黑麦含量（0% 或 20%）和木聚糖酶补充（0 或 200 mg/kg 饲料）作为因素。黑麦的加入增加了十二指肠和回肠隐窝深度、绒毛高度、绒毛与隐窝比率和吸收表面积（ p ％3C 0.05）以及回肠粘膜厚度和隐窝宽度（ p ％3C 0.05）。木聚糖酶补充减弱了黑麦对十二指肠和回肠的影响，并降低了空肠中的绒毛高度和绒毛与隐窝的比率（ p < 0.05）。黑麦和木聚糖酶对紧密蛋白 3 和 ZO-1 蛋白的空间分布没有影响，但木聚糖酶的补充减少了十二指肠和空肠中紧密蛋白 3 的量 ( p < 0.05)。这项研究的结果表明，在肉鸡日粮中添加 20% 的现代杂交黑麦可以改善十二指肠和回肠的结构，但这些效果会因木聚糖酶的补充而减弱。