ABSTRACT

Purpose: Prolonged surgical procedures and some clinical conditions such as surgeries of thoracoabdominal aorta, mesenteric ischemia, cardiopulmonary bypass, strangulated hernias and neonatal necrotizing enterocolitis may cause decreased perfusion and injury of relevant organs and tissues. After reperfusion, injuries may get worse, leading to ischemia–reperfusion (I/R) injury. Reperfusion following arterial clamping allows oxygen to ischemic tissues and produce injury by multiple mechanisms, including neutrophilic infiltration, intracellular adhesion molecules, and generation of reactive oxygen radicals. In this study with the analysis of SOD, MDA and Caspase-3 levels, we aimed to investigate the effect of topiramate on the outcome of I/R occured after abdominal aorta clamping on rats.

Materials and Methods: Totaly 24 Sprague–Dawley male rats were randomly divided into three experimental groups; the control group (n = 8), I/R (n = 8) and I/R+ topiramate (n = 8). Topiramate (100 mg/kg/day); 50 mg/kg (single dose) was administered intraperitoneally after being diluted with saline 5 days before I/R.

Results: The intestinal tissue of the ischemia group displayed hemorrhage, Crypts of Lieberkuhn degeneration, ulceration, vascular congestion and edematous fields as a result of aortic occlusion. We also observed that MDA levels and Caspase-3 positivity increased and SOD levels decreased in the small intestine. However, topiramate administration decreased Crypts of Lieberkuhn degeneration, ulceration, vascular congestion and edematous fields, Caspase-3 positivity, and MDA levels.

Conclusion: Our findings suggest that topiramate is effective against aortic occlusion-induced intestinal injury by reducing oxidative stress and apoptosis.

摘要

目的：延长手术时间和一些临床情况，如胸腹主动脉手术、肠系膜缺血、体外循环、绞窄性疝和新生儿坏死性小肠结肠炎，可能导致相关器官和组织的灌注减少和损伤。再灌注后，损伤可能会变得更糟，导致缺血-再灌注 (I/R) 损伤。动脉钳夹后的再灌注允许氧气进入缺血组织并通过多种机制产生损伤，包括中性粒细胞浸润、细胞内粘附分子和活性氧自由基的产生。在本研究中，通过分析 SOD、MDA 和 Caspase-3 水平，我们旨在研究托吡酯对大鼠腹主动脉钳夹后 I/R 结果的影响。

材料与方法：将24只Sprague-Dawley雄性大鼠随机分为三个实验组；对照组 ( n = 8)、I/R ( n = 8) 和 I/R+ 托吡酯 ( n = 8)。托吡酯 (100 毫克/公斤/天); 在 I/R 前 5 天用生理盐水稀释后，腹腔注射 50 mg/kg（单剂量）。

结果：缺血组的肠组织因主动脉闭塞而出现出血、Lieberkuhn 隐窝变性、溃疡、血管充血和水肿区域。我们还观察到小肠中的 MDA 水平和 Caspase-3 阳性增加而 SOD 水平降低。然而，托吡酯给药降低了 Lieberkuhn 变性隐窝、溃疡、血管充血和水肿区域、Caspase-3 阳性和 MDA 水平。

结论：我们的研究结果表明，托吡酯可通过减少氧化应激和细胞凋亡来有效对抗主动脉闭塞引起的肠道损伤。