Indoor aquatic mesocosms are increasingly used in nanosafety to assess the behavior, fate and impacts of engineered nanomaterials (ENMs) in aquatic environments using relevant exposure scenarios. The robustness of 60L freshwater mesocosm experimentation was tested on the basis of the reusability of the data collected in a database named MESOCOSM regarding mesocosm experiments examining the environmental risks of CeO2 ENMs. We observed a high reliability of the measured variables across replicates. The sensitivity of this mesocosm methodology was evidenced by the contrasted ecosystem responses revealed by a multivariate analysis. We also observed that adding a variability to the dataset up to 15% did not affect the outcome of the analysis of the results. This ability to buffer this variability demonstrates that the indoor aquatic mesocosms are robust tools contributing to the environmental risk assessment of ENMs, and stresses the benefit of reusing the data stored in databases such as MESOCOSM adhering to the Findable, Accessible, Interoperable, Reusable (FAIR) data principles.

中文翻译：

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室内水生介观试验的稳健性和数据可重用性，以评估纳米材料的环境风险

室内水生生物膜被越来越多地用于纳米安全中，以使用相关的暴露场景来评估工程纳米材料（ENM）在水生环境中的行为，命运和影响。基于在名为MESOCOSM的数据库中收集的有关研究CeO2 ENM的环境风险的中观试验的数据的可重用性，对60L淡水中观试验的鲁棒性进行了测试。我们观察到跨重复测量变量的高度可靠性。多变量分析揭示的生态系统响应对比证明了这种中观方法论的敏感性。我们还观察到，向数据集添加最多15％的变异性不会影响结果分析的结果。