The article describes several methods for the synthesis of biocatalysts based on horseradish root peroxidase (EC 1.11.1.7) immobilized on various carriers (magnetic nanoparticles Fe₃O₄, SiO₂, Al₂O₃, hypercrosslinked polystyrene brand MN-100 and Sepabeads EC-HA). The resulting biocatalytic systems can be used for the environmentally safe synthesis of the intermediate product of vitamin E, 2,3,5-trimethylhydroquinone, by oxidizing 2,3,6-trimethylphenol with hydrogen peroxide. The covalent method of enzyme immobilization was used in the work. Chitosan was used as a modifying agent; glutaraldehyde and 3-aminopropyltritoxysilane were used to activate the enzyme carrier. For all biocatalysts, the optimal conditions for substrate oxidation—temperature and pH value (40 °C and pH 6.5)—have been selected. The best results on the oxidation of 2,3,6-trimethylphenol were shown by a biocatalyst based on peroxidase immobilized on magnetic Fe₃O₄ nanoparticles. This biocatalytic system has shown promising in 5 replicate experiments (the initial rate reached 8 μmol/l). The advantage of using magnetic nanoparticles as an enzyme carrier is the ease with which the magnetic biocatalyst can be removed from the reaction mixture.

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中文翻译：

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环保合成维生素E中间体的生物催化剂

本文介绍了几种基于辣根过氧化物酶（EC 1.11.1.7）固定在各种载体（磁性纳米颗粒Fe ₃ O ₄，SiO ₂，Al ₂ O ₃）上的生物催化剂的合成方法。，超交联的聚苯乙烯品牌MN-100和Sepabeads EC-HA）。通过用过氧化氢氧化2,3,6-三甲基苯酚，所得的生物催化体系可用于环境安全地合成维生素E，2,3,5-三甲基对苯二酚的中间产物。在这项工作中使用了固定化酶的共价方法。壳聚糖用作改性剂。戊二醛和3-氨基丙基三苯甲氧基硅烷用于活化酶载体。对于所有生物催化剂，已经选择了底物氧化的最佳条件-温度和pH值（40°C和pH 6.5）。基于固定在磁性Fe ₃ O ₄上的过氧化物酶的生物催化剂显示出对2,3,6-三甲基苯酚氧化的最佳结果纳米粒子。该生物催化系统在5个重复实验中显示出良好的前景（初始速率达到8μmol/ l）。使用磁性纳米颗粒作为酶载体的优点是易于从反应混合物中除去磁性生物催化剂。

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