Abstract

The present study was planned with the objective to isolate and characterize a carbendazim-degrading Achromobacter sp. strain GB61 from the contaminated agriculture field. Optimized carbendazim biodegradation (76.2%) by Achromobacter sp. strain GB61 was observed at 30 °C, pH 7.0, and 120 rpm within 20 days of incubation and was determined using response surface methodology. The degradation kinetics revealed the half-life (t_1/2) of 7.3 days, and correlation coefficient (R²) 0.0882 and degradation constant (K) 0.095 respectively. Maximum specific degradation rate (q_max), half-saturation constant (K_s) and inhibition constant (K_i) were determined to be 0.122 d⁻¹, 0.724 mg L⁻¹ and 271.71 mg L⁻¹, respectively. Intermediate metabolites of carbendazim biodegradation confirming the formation of dihydroxybenzimidazole, 2-aminobenzimidazole and benzimidazole as intermediates. The results of the experiments suggested that the strain GB61 could be used for large-scale bioremediation of carbendazim contaminated environments.

摘要

计划本研究的目的是分离和表征可降解多菌灵的无色杆菌。来自受污染农田的菌株 GB61。无色杆菌对多菌灵生物降解的优化 (76.2%) 。在 30 °C、pH 7.0 和 120 rpm 的孵育 20 天内观察到菌株 GB61，并使用响应面法确定。降解动力学显示半衰期( t _1/2 )为7.3天，相关系数( R ² )为0.0882，降解常数( K )分别为0.095。最大比降解率 ( q _max )，半饱和常数 ( K _s) 和抑制常数 ( K _i ) 分别确定为 0.122 d ^-1、0.724 mg L ^-1和 271.71 mg L ^-1。多菌灵生物降解的中间代谢物证实形成二羟基苯并咪唑、2-氨基苯并咪唑和苯并咪唑作为中间体。实验结果表明，GB61菌株可用于多菌灵污染环境的大规模生物修复。